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  • Featured Product
  • KD/KO Validated

Glucocorticoid receptor Polyclonal antibody

Glucocorticoid receptor Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 24050-1-AP

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    WB analysis using 24050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    WB analysis using 24050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    WB analysis of HepG2 using 24050-1-AP

    WB result of Glucocorticoid receptor antibody (24050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glucocorticoid receptor transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    WB analysis using 24050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    WB analysis using 24050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    WB analysis using 24050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IP experiment of HepG2 using 24050-1-AP

    IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IP experiment of HepG2 using 24050-1-AP

    IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IP experiment of mouse heart using 24050-1-AP

    IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:500) with mouse heart tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IHC staining of human prostate cancer using 24050-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IHC staining of human prostate cancer using 24050-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IHC staining of human ovary tumor using 24050-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IHC staining of human ovary tumor using 24050-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IF Staining of A549 using 24050-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IF Staining of HepG2 using 24050-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Glucocorticoid receptor Polyclonal antibody (24050-1-AP)

    IF Staining of HepG2 using 24050-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells
Positive IP detected inHepG2 cells, mouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24050-1-AP targets Glucocorticoid receptor in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Rare minnow
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 94-97 kDa
GenBank Accession NumberBC015610
Gene Symbol NR3C1
Gene ID (NCBI) 2908
RRIDAB_2813890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

Protocols

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IHC protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IF protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IP protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.

Authors - Wenxiang Hu
View Article
mouseIHC

Cell Metab

A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.

Authors - Anne Loft
View Article
humanWB

Sci Adv

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Authors - Leiming Wang
View Article
mousechIP

Mol Cell

Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.

Authors - Fabiana Quagliarini
View Article
mouseWB,ChIP,IP

J Clin Invest

REVERBa couples the circadian clock to hepatic glucocorticoid action.

Authors - Giorgio Caratti
View Article
mouseChIP

Mol Psychiatry

The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.

Authors - Alexander S Häusl
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

  • Applications: Western Blot, Immunoprecipitation,
  • Cell Tissue Type: mouse brain tissue
FH

Yu Siong (Verified Customer) (04-23-2019)

Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Porcine brain endothelial cells
FH

Praveen (Verified Customer) (02-25-2019)

WORKS VERY WELL.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Cell culture, ChIP,
  • Cell Tissue Type: Ac16
FH

Shubham (Verified Customer) (01-31-2019)

Antibody works great.

  • Applications: Western Blot, Immunohistochemistry, Cell culture,
  • Primary Antibody Dilution: 1:10
  • Cell Tissue Type: Ac16
Glucocorticoid receptor Antibody Western Blot,Immunohistochemistry,Cell culture, validation (1:10 dilution) in Ac16 (Cat no:24050-1-AP)