• Featured Product
  • KD/KO Validated

Glucocorticoid receptor Antibody

Glucocorticoid receptor Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 24050-1-AP

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, A431 cells
Positive IP detected inmouse heart tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 24050-1-AP immunogen is Fusion Protein Glucocorticoid receptor protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, Rare minnows, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated molecular weight 86 kDa
Observed molecular weight 94-97 kDa
GenBank accession number BC015610
Gene symbol NR3C1
Gene ID (NCBI) 2908
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

Protocols

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IF protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IP protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP,chIP

Nat Commun

E47 modulates hepatic glucocorticoid action.

Authors - M Charlotte Hemmer
mouseIP

J Mol Endocrinol

An improved method for quantitative ChIP studies of nuclear receptor function.

Authors - Ann Louise Hunter
mouseWB,IF

Cells

Cortisol Excess-Mediated Mitochondrial Damage Induced Hippocampal Neuronal Apoptosis in Mice Following Cold Exposure.

Authors - Bin Xu
mouseWB,IF

Animals (Basel)

Corticosterone Excess-Mediated Mitochondrial Damage Induces Hippocampal Neuronal Autophagy in Mice Following Cold Exposure.

Authors - Bin Xu
humanWB

Sci Adv

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Authors - Leiming Wang
humanWB

Med Oncol

α-Viniferin activates autophagic apoptosis and cell death by reducing glucocorticoid receptor expression in castration-resistant prostate cancer cells.

Authors - Kejun Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

  • Applications: Western Blot,Immunoprecipitation,
  • Cell Tissue Type: mouse brain tissue
No image submitted with review
FH

Yu Siong (Verified Customer) (04-23-2019)

Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur. Consistent in two Independent experiments

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Porcine brain endothelial cells
No image submitted with review
FH

Praveen (Verified Customer) (02-25-2019)

WORKS VERY WELL.

  • Applications: Western Blot,Immunohistochemistry,Immunofluorescence,Cell culture,ChIP,
  • Cell Tissue Type: Ac16
No image submitted with review
FH

Shubham (Verified Customer) (01-31-2019)

Antibody works great.

  • Applications: Western Blot,Immunohistochemistry,Cell culture,
  • Primary Antibody Dilution: 1:10
  • Cell Tissue Type: Ac16
Glucocorticoid receptor Antibody Western Blot,Immunohistochemistry,Cell culture, validation (1:10 dilution) in Ac16 (Cat no:24050-1-AP)