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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Glucocorticoid receptor Polyclonal antibody

Glucocorticoid receptor Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-24050

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated Glucocorticoid receptor Po (CL594-24050)

    IF Staining of A549 using CL594-24050

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 Glucocorticoid receptor antibody (CL594-24050) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Glucocorticoid receptor Po (CL594-24050)

    FC experiment of HeLa using CL594-24050

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Glucocorticoid receptor (CL594-24050) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA549 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-24050 is Glucocorticoid receptor Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 94-97 kDa
GenBank Accession NumberBC015610
Gene Symbol NR3C1
Gene ID (NCBI) 2908
RRIDAB_2919872
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

Protocols

Product Specific Protocols
IF protocol for CL594 Glucocorticoid receptor antibody CL594-24050Download protocol
FC protocol for CL594 Glucocorticoid receptor antibody CL594-24050Download protocol
Standard Protocols
Click here to view our Standard Protocols