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  • KD/KO Validated

NSDHL Antibody

NSDHL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15111-1-AP

Synonyms

H105E3, NSDHL, Protein H105e3, SDR31E1, XAP104



Tested Applications

Positive WB detected inHeLa cells, human heart tissue, mouse brain tissue
Positive IHC detected inhuman skin cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human lung tissue, human lung cancer tissue, human ovary tissue, human ovary tumor tissue, human stomach tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15111-1-AP targets NSDHL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSDHL fusion protein Ag7097
Full Name NAD(P) dependent steroid dehydrogenase-like
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa,40 kDa
GenBank accession number BC000245
Gene symbol NSDHL
Gene ID (NCBI) 50814
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).

Protocols

Product Specific Protocols
WB protocol for NSDHL antibody 15111-1-APDownload protocol
IHC protocol for NSDHL antibody 15111-1-APDownload protocol
IF protocol for NSDHL antibody 15111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis.

Authors - Monika Lewinska
humanIHC

Mol Cancer Ther

EGFR and RB1 as Dual Biomarkers in HPV-Negative Head and Neck Cancer.

Authors - Tim N Beck
mouseWB, IHC

Cell Rep

Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.

Authors - Linara Gabitova
mouseWB,IHC

Clin Cancer Res

Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.

Authors - Renata E Gordon
  • KD Validated
mouseIHC

Cancer Discov

Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation.

Authors - Sukhanova Anna A