- Featured Product
- KD/KO Validated
NSDHL Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 15111-1-AP
H105E3, NSDHL, Protein H105e3, SDR31E1, XAP104
|Positive WB detected in||HeLa cells, human heart tissue, mouse brain tissue|
|Positive IHC detected in||human skin cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human lung tissue, human lung cancer tissue, human ovary tissue, human ovary tumor tissue, human stomach tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3200|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15111-1-AP targets NSDHL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NSDHL fusion protein Ag7097|
|Full Name||NAD(P) dependent steroid dehydrogenase-like|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||42 kDa,40 kDa|
|GenBank accession number||BC000245|
|Gene ID (NCBI)||50814|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).
Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis.
Mol Cancer Ther
EGFR and RB1 as Dual Biomarkers in HPV-Negative Head and Neck Cancer.
Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.
Clin Cancer Res
Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.
Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation.