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NUDT19 Polyclonal antibody

NUDT19 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55126-1-AP

Synonyms

Nudix motif 19, NUDT19, RP2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NUDT19 Polyclonal antibody (55126-1-AP)

    WB analysis of HEK-293 using 55126-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55126-1-AP (NUDT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NUDT19 Polyclonal antibody (55126-1-AP)

    WB analysis of HEK-293 using 55126-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55126-1-AP (NUDT19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NUDT19 Polyclonal antibody (55126-1-AP)

    WB analysis of HEK-293 using 55126-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55126-1-AP (NUDT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUDT19 Polyclonal antibody (55126-1-AP)

    IHC staining of human kidney using 55126-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUDT19 Polyclonal antibody (55126-1-AP)

    IHC staining of human kidney using 55126-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NUDT19 Polyclonal antibody (55126-1-AP)

    IHC staining of human liver using 55126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NUDT19 Polyclonal antibody (55126-1-AP)

    IHC staining of human liver using 55126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NUDT19 Polyclonal antibody (55126-1-AP)

    IHC staining of human liver using 55126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NUDT19 Polyclonal antibody (55126-1-AP)

    IHC staining of human liver using 55126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55126-1-AP targets NUDT19 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 19
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberNM_001105570
Gene Symbol NUDT19
Gene ID (NCBI) 390916
RRIDAB_10858394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT19, also named as RP2, belongs to the Nudix hydrolase family. It is a coenzyme A diphosphatase that mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters. The antibody is specific to NUDT19.

Protocols

Product Specific Protocols
WB protocol for NUDT19 antibody 55126-1-APDownload protocol
IHC protocol for NUDT19 antibody 55126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols