|Positive WB detected in||mouse kidney tissue, mouse liver tissue, human kidney tissue, human liver tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:800|
|Sample-dependent, check data in validation data gallery|
13409-1-AP targets Occludin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||cow, human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Occludin fusion protein Ag4057|
|Calculated molecular weight||522 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC029886|
|Gene ID (NCBI)||4950|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Amy (Verified Customer) (05-22-2019)
I approached Proteintech for guidance with regards to dilutions of this antibody, and with their help I optimized the protocol in good time. Very helpful!
JIMMY (Verified Customer) (03-26-2019)
This antibody offers a good detection of Occludin on mouse brain endothelial cell line, bEnd3 cells, through IHC.