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Occludin Antibody

Occludin Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13409-1-AP

Synonyms

occludin, OCLN



Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue, human kidney tissue, human liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:800
Sample-dependent, check data in validation data gallery

Product Information

13409-1-AP targets Occludin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity cow, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Occludin fusion protein Ag4057
Full Name occludin
Calculated molecular weight 522aa,59 kDa
Observed molecular weight 59 kDa
GenBank accession number BC029886
Gene symbol OCLN
Gene ID (NCBI) 4950
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 13409-1-APDownload protocol
IHC protocol for Occludin antibody 13409-1-APDownload protocol
IP protocol for Occludin antibody 13409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
swineWB

PeerJ

Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets.

Authors - Shiqiong Wang
humanWB

Lab Invest

Glucagon-like peptide-1 receptor activation alleviates lipopolysaccharide-induced acute lung injury in mice via maintenance of endothelial barrier function.

Authors - Jian Xu
mouseWB

Nat Commun

Intestinal non-canonical NFκB signaling shapes the local and systemic immune response.

Authors - Sadeesh K Ramakrishnan
humanWB

Front Microbiol

A Novel Postbiotic From Lactobacillus rhamnosus GG With a Beneficial Effect on Intestinal Barrier Function.

Authors - Jie Gao
mouseWB

Biochem Biophys Res Commun

Bacterial β-glucuronidase alleviates dextran sulfate sodium-induced colitis in mice: A possible crucial new diagnostic and therapeutic target for inflammatory bowel disease.

Authors - Yan He
humanWB

Ren Fail

Cadmium regulates von Willebrand factor and occludin expression in glomerular endothelial cells of mice in a TNF-α-dependent manner.

Authors - Zongguo Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amy (Verified Customer) (05-22-2019)

I approached Proteintech for guidance with regards to dilutions of this antibody, and with their help I optimized the protocol in good time. Very helpful!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse Colon
No image submitted with review
FH

JIMMY (Verified Customer) (03-26-2019)

This antibody offers a good detection of Occludin on mouse brain endothelial cell line, bEnd3 cells, through IHC.

  • Applications: Immunofluorescence,
No image submitted with review