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  • KD/KO Validated

OMA1 Polyclonal antibody

OMA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17116-1-AP

Synonyms

2010001O09Rik, DAB1, MPRP 1, MPRP1, OMA1, YKR087C, ZMPOMA1



Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, K-562 cells, HepG2 cells, SKOV-3 cells, MDA-MB-231 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

17116-1-AP targets OMA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OMA1 fusion protein Ag10803
Full Name OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
Calculated molecular weight 524 aa, 60 kDa
Observed molecular weight34, 60 kDa
GenBank accession numberBC012915
Gene symbol OMA1
Gene ID (NCBI) 115209
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OMA1 (Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1 (Metalloprotease-related protein 1) and belongs to the peptidase M48 family. It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants, the 40 kDa form of OMA1 may be the mature form (M-OMA1), and the 35 kDa is a cleaved form (S-OMA1), with the 60 kDa form being the active enzyme (PMID:20334834, 30518622). This antibody is speicific to OMA1.

Protocols

Product Specific Protocols
WB protocol for OMA1 antibody 17116-1-APDownload protocol
IHC protocol for OMA1 antibody 17116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Authors - Evelyn Fessler
mouseWB

Redox Biol

Peroxiredoxin 3 deficiency induces cardiac hypertrophy and dysfunction by impaired mitochondrial quality control.

Authors - Seong Keun Sonn
  • KD Validated
humanWB

Cell Rep

The Genomic Landscape of Renal Oncocytoma Identifies a Metabolic Barrier to Tumorigenesis.

Authors - Shilpy Joshi
mouseWB

Sci Total Environ

Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice.

Authors - Guo-Xiang Zhou
human,mouseWB

Hum Mol Genet

Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.

Authors - Yi-Ting Liu
  • KO Validated
mouseWB

Neuropharmacology

The adenosine A2A receptor antagonist protects against retinal mitochondrial injury in association with an altered network of competing endogenous RNAs.

Authors - Gengjing Fang