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Occludin Monoclonal antibody

Occludin Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D3C4

Cat no : 66378-1-Ig

Synonyms

Occludin, OCLN



Tested Applications

Positive WB detected inHUVEC cells, Jurkat cells, mouse colon tissue, rat colon tissue, 4T1 cells, pig colon tissue
Positive IHC detected inhuman colon cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

66378-1-Ig targets Occludin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Occludin fusion protein Ag4057
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC029886
Gene symbol OCLN
Gene ID (NCBI) 4950
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 66378-1-IgDownload protocol
IHC protocol for Occludin antibody 66378-1-IgDownload protocol
IF protocol for Occludin antibody 66378-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acta Cir Bras

Hydroxysafflor yellow A acutely attenuates blood-brain barrier permeability, oxidative stress, inflammation and apoptosis in traumatic brain injury in rats1.

Authors - Jianjun Xu
pigWB

J Anim Sci

Effects and interaction of dietary electrolyte balance and citric acid on the intestinal function of weaned piglets.

Authors - Qingqing Deng
WB,IF

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang
pigWB

Front Physiol

Taurine Alleviates Intestinal Injury by Mediating Tight Junction Barriers in Diquat-Challenged Piglet Models.

Authors - Chaoyue Wen
ratIF

Drug Des Devel Ther

Paeoniflorin Alleviates Abnormalities in Rats with Functional Dyspepsia by Stimulating the Release of Acetylcholine.

Authors - Xuan Zou
humanIF

Ann Transl Med

Maternally expressed 3 protects the intestinal barrier from cardiac arrest-induced ischemia/reperfusion injury via miR-34a-3p/sirtuin 1/nuclear factor kappa B signaling.

Authors - Xianwei Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Doriane (Verified Customer) (08-25-2019)

Satisfying antibody used for automated Western-blot Wes (Protein Simple)

  • Applications: Western Blot,Other,
  • Primary Antibody Dilution: Automated Western Blot Wes 1/400
  • Cell Tissue Type: Caco-2/TC7 cell line