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Occludin Antibody

Occludin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D3C4

Cat no : 66378-1-Ig

Synonyms

Occludin, OCLN



Tested Applications

Positive WB detected inHUVEC cells, 4T1 cells, Jurkat cells, mouse colon tissue, pig colon tissue, rat colon tissue
Positive IHC detected inhuman colon cancer tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66378-1-Ig targets Occludin in IHC, WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Reactivity human, mouse, rat, pig
Cited Species mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Occludin fusion protein Ag4057
Full Name occludin
Calculated molecular weight 522aa,59 kDa
Observed molecular weight 59 kDa
GenBank accession number BC029886
Gene symbol OCLN
Gene ID (NCBI) 4950
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 66378-1-IgDownload protocol
IHC protocol for Occludin antibody 66378-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

J Anim Sci

Effects and interaction of dietary electrolyte balance and citric acid on the intestinal function of weaned piglets.

Authors - Qingqing Deng
WB,IF

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang
mouseWB,IF

Front Pharmacol

Huang-Lian-Jie-Du Decoction Ameliorates Acute Ulcerative Colitis in Mice via Regulating NF-κB and Nrf2 Signaling Pathways and Enhancing Intestinal Barrier Function.

Authors - Ziwen Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Doriane Aguanno (08-25-2019)

Satisfying antibody used for automated Western-blot Wes (Protein Simple)

  • Applications: Western Blot,Other,
  • Primary Antibody Dilution: Automated Western Blot Wes 1/400
  • Cell Tissue Type: Caco-2/TC7 cell line
No image submitted with review