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Occludin Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66378-1-Ig |CloneNo.: 1D3C4

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Occludin Antibody WB HUVEC cells 66378-1-Ig

HUVEC cells were subjected to SDS PAGE followed by western blot with 66378-1-Ig( Occludin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Occludin Antibody WB Jurkat cells 66378-1-Ig

WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells

Occludin Antibody IHC human colon cancer tissue 66378-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Occludin Antibody IHC human colon cancer tissue 66378-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
HUVEC cells, Jurkat cells, pig colon tissue, 4T1 cells
Positive IHC detected in:
human colon cancer tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Occludin fusion protein Ag4057
Full name:
occludin
Calculated molecular weight:
522aa,59 kDa
Observed molecular weight:
59 kDa
GenBank accession number:
BC029886
Gene ID (NCBI):
4950
Gene symbol
OCLN
Synonyms
Occludin, OCLN

Background

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).


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