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Occludin Monoclonal antibody
Occludin Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
1D3C4
Cat no : 66378-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, Jurkat cells, mouse colon tissue, rat colon tissue, 4T1 cells, pig colon tissue |
| Positive IHC detected in | human colon cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:800-1:3200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 5 publications below |
| IF | See 4 publications below |
Product Information
66378-1-Ig targets Occludin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Occludin fusion protein Ag4057 |
| Full Name | occludin |
| Calculated molecular weight | 522 aa, 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC029886 |
| Gene symbol | OCLN |
| Gene ID (NCBI) | 4950 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Occludin antibody 66378-1-Ig | Download protocol |
| IHC protocol for Occludin antibody 66378-1-Ig | Download protocol |
| IF protocol for Occludin antibody 66378-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Cir Bras Hydroxysafflor yellow A acutely attenuates blood-brain barrier permeability, oxidative stress, inflammation and apoptosis in traumatic brain injury in rats1. | ||
J Anim Sci Effects and interaction of dietary electrolyte balance and citric acid on the intestinal function of weaned piglets. | ||
Theriogenology Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway. | ||
Front Physiol Taurine Alleviates Intestinal Injury by Mediating Tight Junction Barriers in Diquat-Challenged Piglet Models. | ||
Drug Des Devel Ther Paeoniflorin Alleviates Abnormalities in Rats with Functional Dyspepsia by Stimulating the Release of Acetylcholine. | ||
Ann Transl Med Maternally expressed 3 protects the intestinal barrier from cardiac arrest-induced ischemia/reperfusion injury via miR-34a-3p/sirtuin 1/nuclear factor kappa B signaling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Doriane (Verified Customer) (08-25-2019) | Satisfying antibody used for automated Western-blot Wes (Protein Simple)
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