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Occludin Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66378-1-Ig |CloneNo.: 1D3C4

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Occludin Antibody WB HUVEC cells 66378-1-Ig

HUVEC cells were subjected to SDS PAGE followed by western blot with 66378-1-Ig( Occludin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Occludin Antibody WB Jurkat cells 66378-1-Ig

WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells

Occludin Antibody IHC human colon cancer tissue 66378-1-Ig

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Occludin Antibody IHC human colon cancer tissue 66378-1-Ig

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
HUVEC cells, Jurkat cells, pig colon tissue, 4T1 cells
Positive IHC detected in:
human colon cancer tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Occludin fusion protein Ag4057
Full name:
occludin
Calculated molecular weight:
522aa,59 kDa
Observed molecular weight:
59 kDa
GenBank accession number:
BC029886
Gene ID (NCBI):
4950
Gene symbol
OCLN
Synonyms
Occludin, OCLN

Background

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels (PMID: 22083955).


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