Occludin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Occludin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D3C4

Cat no : 66378-1-PBS

Synonyms

Occludin, OCLN

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Validation Data Gallery

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  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66378-1-Ig (same clone as 66378-1-PBS)

  WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66378-1-Ig (same clone as 66378-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 66378-1-Ig (same clone as 66378-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (66378-1-Ig, Clone: 1D3C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Occludin antibody (66378-1-Ig, Clone: 1D3C4 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66378-1-PBS targets Occludin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Occludin fusion protein Ag4057
• Full Name: occludin
• Calculated Molecular Weight: 522 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC029886
• Gene Symbol: OCLN
• Gene ID (NCBI): 4950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).