|Positive WB detected in||HEK-293 cells, PC-3 cells, HeLa cells, HEK293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
10883-1-AP targets P16-INK4A in WB, IP, FC, CoIP,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P16-INK4A fusion protein Ag1328|
|Full Name||cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16-18 kDa|
|GenBank accession number||BC021998|
|Gene ID (NCBI)||1029|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.
What is the molecular weight of P16?
16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.
What is the function of p16?
P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).
What is the role of p16 in senescence?
In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.
What is the role of p16 in cancer?
As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).
|Product Specific Protocols|
|WB protocol for P16-INK4A antibody 10883-1-AP||Download protocol|
|IF protocol for P16-INK4A antibody 10883-1-AP||Download protocol|
|IP protocol for P16-INK4A antibody 10883-1-AP||Download protocol|
|FC protocol for P16-INK4A antibody 10883-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways.
Involvement of Lamin B1 Reduction in Accelerated Cellular Senescence during Chronic Obstructive Pulmonary Disease Pathogenesis.
Aging (Albany NY)
lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs.
Aging (Albany NY)
S100A13 promotes senescence-associated secretory phenotype and cellular senescence via modulation of non-classical secretion of IL-1α.
Cell Death Dis
p53 induces senescence through Lamin A/C stabilization-mediated nuclear deformation.
Free Radic Biol Med
Sirt3 mediates the protective effect of hydrogen in inhibiting ROS-induced retinal senescence.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joshua (Verified Customer) (03-12-2020)
MDCKs fixed in 4% paraformaldehyde and stained overnight at 4 C. Excellent, bright stain.
Kyle (Verified Customer) (02-28-2019)
Clean bands, low background