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P16-INK4A Antibody 57 Publications

Rabbit Polyclonal| Catalog number: 10883-1-AP

Featured Product KD/KO validated

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Con: 55 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, CoIP, ELISA
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Images See all

P16-INK4A Antibody WB HEK-293 cells 10883-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

P16-INK4A Antibody WB HEK293 cells 10883-1-AP

WB result of p16-INK4A antibody (10883-1-AP, 1:2,000) with si-Control and si-p16 transfected HEK-293 cells.

P16-INK4A Antibody IHC human cervical cancer tissue 10883-1-AP

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP( P16-INK4A Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P16-INK4A Antibody IHC human cervical cancer tissue 10883-1-AP

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP( P16-INK4A Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P16-INK4A Antibody IP HEK-293 cells 10883-1-AP

IP Result of anti-P16 (IP:10883-1-AP, 4ug; Detection:10883-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

P16-INK4A Antibody IF SH-SY5Y cells 10883-1-AP

Immunofluorescent analysis of SH-SY5Y cells, using CDKN2A antibody 10883-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

P16-INK4A Antibody FC HeLa cells 10883-1-AP

1X10^6 HeLa cells were stained with .2ug P16-INK4A antibody (10883-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, monkey
Positive WB detected in:
HEK-293 cells, PC-3 cells, HeLa cells, HEK293 cells
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human cervical cancer tissue, human lung cancer tissue, human lymphoma tissue, human tonsillitis tissue
Positive IF detected in:
SH-SY5Y cells, HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
P16-INK4A fusion protein Ag1328
Full name:
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Calculated molecular weight:
16 kDa
Observed molecular weight:
16-18 kDa
GenBank accession number:
BC021998
Gene ID (NCBI):
1029
Gene symbol
CDKN2A
Synonyms
ARF, ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, TP16

Background

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. The third one -p14(ARF) is entirely unrelated. 10883-1-AP reacts with p16 specifically. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma.


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