Name: P16-INK4A Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, FC, CoIP, ELISA
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P16-INK4A Antibody WB HEK-293 cells 10883-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

WB result of p16-INK4A antibody (10883-1-AP, 1:2,000) with si-Control and si-p16 transfected HEK-293 cells.

P16-INK4A Antibody IP HEK-293 cells 10883-1-AP

IP Result of anti-P16 (IP:10883-1-AP, 4ug; Detection:10883-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

P16-INK4A Antibody FC HeLa cells 10883-1-AP

1X10^6 HeLa cells were stained with .2ug P16-INK4A antibody (10883-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:

human, monkey

Positive WB detected in:

HEK-293 cells, PC-3 cells, HeLa cells, HEK293 cells

Positive IP detected in:

HEK-293 cells

Positive FC detected in:

HeLa cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

P16-INK4A fusion protein Ag1328

Full name:

cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Calculated molecular weight:

16 kDa

Observed molecular weight:

16-18 kDa

GenBank accession number:

BC021998

Gene ID (NCBI):

1029

Gene symbol

CDKN2A

Synonyms

ARF, ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, TP16

Background

Background

p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.

What is the molecular weight of P16?

16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.

What is the function of p16?

P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).

What is the role of p16 in senescence?

In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.

What is the role of p16 in cancer?

As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).

Customer Reviews

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P16-INK4A Rabbit Polyclonal antibody

Kyle (Verified Customer), 02-28-2019

Applications

Western Blot,

Primary Antibody Dilution

1:1000

No image submitted with review

Cell/Tissue Type

small cell lung cancer

Lot Number

Clean bands, low background

Showing 1- [6] of [76] Publications

Author:		Motterle Anna A
Pubmed ID:		22706276
Journal:		Hum Mol Genet
Title:		Functional analyses of coronary artery disease associated variation on chromosome 9p21 in vascular smooth muscle cells.
Application:		IHC

Species:		human

Author:		Wang Fan F
Pubmed ID:		22986536
Journal:		Biotechnol Lett
Title:		Notch1 signaling contributes to the oncogenic effect of HBx on human hepatic cells.
Application:		WB

Species:		human

Author:		Xu Xihui X
Pubmed ID:		23583271
Journal:		J Hepatol
Title:		Deficiency of insulin-like growth factor 1 attenuates aging-induced changes in hepatic function: Role of autophagy.
Application:		WB

Species:		human, mouse

Author:		Deng Shan S
Pubmed ID:		23758730
Journal:		BMC Complement Altern Med
Title:		Teng-Long-Bu-Zhong-Tang, a Chinese herbal formula, enhances anticancer effects of 5 - Fluorouracil in CT26 colon carcinoma.
Application:		WB,IHC

Species:		mouse

Author:		Chidlow Glyn G
Pubmed ID:		24069379
Journal:		PLoS One
Title:		Ocular Expression and Distribution of Products of the POAG-Associated Chromosome 9p21 Gene Region.
Application:		WB

Species:		rat

Author:		Peir-In Liang
Pubmed ID:		24114011
Journal:		Tumour Biol
Title:		BCL6 overexpression is associated with decreased p19(ARF) expression and confers an independent prognosticator in gallbladder carcinoma.
Application:		IHC

Species:

WB Figures

WB result of p16-INK4A antibody (10883-1-AP, 1:2,000) with si-Control and si-p16 transfected HEK-293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

P16-INK4A Antibody WB HeLa cells 10883-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

P16-INK4A Antibody WB PC-3 cells 10883-1-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

P16-INK4A Antibody IHC human lung cancer tissue 10883-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10883-1-AP( P16-INK4A Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10883-1-AP( P16-INK4A Antibody) at dilution of 1:200 (under 40x lens).

P16-INK4A Antibody IHC human lymphoma tissue 10883-1-AP

Immunohistochemical of paraffin-embedded human lymphoma using 10883-1-AP(P16 antibody) at dilution of 1:100 (under 40x lens)

P16-INK4A Antibody IHC human tonsillitis tissue 10883-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10883-1-AP( P16-INK4A Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10883-1-AP( P16-INK4A Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Figures

IP Result of anti-P16 (IP:10883-1-AP, 4ug; Detection:10883-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

IF Figures

P16-INK4A Antibody IF HeLa cells 10883-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 10883-1-AP (P16-INK4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

FC Figures

1X10^6 HeLa cells were stained with 0.2ug P16-INK4A antibody (10883-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

P16-INK4A Antibody 76 Publications

Rabbit Polyclonal| Catalog number: 10883-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

P16-INK4A Rabbit Polyclonal antibody

Showing 1- [6] of [76] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

FC Figures

P16-INK4A Antibody 76 Publications

Rabbit Polyclonal| Catalog number: 10883-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

P16-INK4A Rabbit Polyclonal antibody