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  • KD/KO Validated

P16-INK4A Polyclonal antibody

P16-INK4A Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10883-1-AP

Synonyms

ARF, ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, TP16



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, HeLa cells, HEK293 cells
Positive IP detected inHEK-293 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

10883-1-AP targets P16-INK4A in WB, IP, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P16-INK4A fusion protein Ag1328
Full Name cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Calculated molecular weight 16 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberBC021998
Gene symbol CDKN2A
Gene ID (NCBI) 1029
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Background

p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.

 What is the molecular weight of P16?

16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.

What is the function of p16?

P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).

 What is the role of p16 in senescence?

In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.

 What is the role of p16 in cancer?

As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).


Protocols

Product Specific Protocols
WB protocol for P16-INK4A antibody 10883-1-APDownload protocol
IF protocol for P16-INK4A antibody 10883-1-APDownload protocol
IP protocol for P16-INK4A antibody 10883-1-APDownload protocol
FC protocol for P16-INK4A antibody 10883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways.

Authors - Yu-An Chen
humanWB

J Immunol

Involvement of Lamin B1 Reduction in Accelerated Cellular Senescence during Chronic Obstructive Pulmonary Disease Pathogenesis.

Authors - Nayuta Saito
humanWB

Aging (Albany NY)

lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs.

Authors - Xiao Lin
humanWB

Aging (Albany NY)

S100A13 promotes senescence-associated secretory phenotype and cellular senescence via modulation of non-classical secretion of IL-1α.

Authors - Yuanyuan Su
humanWB

Cell Death Dis

p53 induces senescence through Lamin A/C stabilization-mediated nuclear deformation.

Authors - Min-Ho Yoon
mouseWB

Free Radic Biol Med

Sirt3 mediates the protective effect of hydrogen in inhibiting ROS-induced retinal senescence.

Authors - Ruichan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (03-12-2020)

MDCKs fixed in 4% paraformaldehyde and stained overnight at 4 C. Excellent, bright stain.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MDCK
P16-INK4A Antibody Immunofluorescence, validation (1:500 dilution) in MDCK (Cat no:10883-1-AP)
FH

Kyle (Verified Customer) (02-28-2019)

Clean bands, low background

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: small cell lung cancer