DDX6 Polyclonal antibody

DDX6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14632-1-AP

Synonyms

ATP dependent RNA helicase p54, DDX6, DEAD box protein 6, HLR2, Oncogene RCK, P54, RCK



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14632-1-AP targets DDX6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX6 fusion protein Ag6211
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC065007
Gene symbol DDX6
Gene ID (NCBI) 1656
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P54, also named as DDX6, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

Protocols

Product Specific Protocols
WB protocol for DDX6 antibody 14632-1-APDownload protocol
IHC protocol for DDX6 antibody 14632-1-APDownload protocol
IF protocol for DDX6 antibody 14632-1-APDownload protocol
IP protocol for DDX6 antibody 14632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biol Reprod

Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development.

Authors - Chappell Vesna A VA