Name: PARK2/Parkin Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee

PARK2/Parkin Antibody WB mouse brain tissue 14060-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14060-1-AP( PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

PARK2/Parkin Antibody IHC mouse brain tissue 14060-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PARK2/Parkin Antibody IF SH-SY5Y cells 14060-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 14060-1-AP(PARK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat

Positive WB detected in:

mouse brain tissue, C6 cells, PC-12 cells, SH-SY5Y cells

Positive IHC detected in:

mouse brain tissue

Positive IF detected in:

SH-SY5Y cells

Recommended dilution:

WB : 1:200-1:1000

IHC : 1:100-1:400

IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

PARK2/Parkin fusion protein Ag5092

Full name:

Parkinson disease (autosomal recessive, juvenile) 2, parkin

Calculated molecular weight:

52 kDa

Observed molecular weight:

52 kDa

GenBank accession number:

BC022014

Gene ID (NCBI):

5071

Gene symbol

PARK2

Synonyms

AR JP, LPRS2, PARK2, Parkin, Parkinson disease protein 2, PDJ, PRKN

Background

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin–protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson’s disease. It has 8 isoforms produced by alternative splicing.

Showing 1- [2] of [2] Publications

Author:		Qichao Huang
Pubmed ID:		27124102
Journal:		Autophagy
Title:		Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.
Application:		WB

Species:		human

Author:		Wei Wang
Pubmed ID:		29091860
Journal:		Biomed Pharmacother
Title:		Eclalbasaponin I from Aralia elata (Miq.) Seem. reduces oxidative stress-induced neural cell death by autophagy activation.
Application:		WB

Species:		human

WB Figures

PARK2/Parkin Antibody WB C6 cells 14060-1-AP

C6 cells were subjected to SDS PAGE followed by western blot with 14060-1-AP( PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14060-1-AP( PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

PARK2/Parkin Antibody WB PC-12 cells 14060-1-AP

PC-12 cells were subjected to SDS PAGE followed by western blot with 14060-1-AP( PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

PARK2/Parkin Antibody WB SH-SY5Y cells 14060-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14060-1-AP( PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IF Figures

Immunofluorescent analysis of ( 4% PFA) fixed SH-SY5Y cells using 14060-1-AP (PARK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 14060-1-AP(PARK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PARK2/Parkin Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 14060-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [2] of [2] Publications

WB Figures

IHC Figures

IF Figures

PARK2/Parkin Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 14060-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background