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PARK2/Parkin Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 14060-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

PARK2/Parkin Antibody WB C6 cells 14060-1-AP

C6 cells were subjected to SDS PAGE followed by western blot with 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

PARK2/Parkin Antibody IHC mouse brain tissue 14060-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PARK2/Parkin Antibody IHC mouse brain tissue 14060-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PARK2/Parkin Antibody IF SH-SY5Y cells 14060-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 14060-1-AP(PARK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
C6 cells, SH-SY5Y cells, PC-12 cells, mouse brain tissue
Positive IHC detected in:
mouse brain tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PARK2/Parkin fusion protein Ag5092
Full name:
Parkinson disease (autosomal recessive, juvenile) 2, parkin
Calculated molecular weight:
52 kDa
Observed molecular weight:
52 kDa
GenBank accession number:
BC022014
Gene ID (NCBI):
5071
Gene symbol
PARK2
Synonyms
AR JP, LPRS2, PARK2, Parkin, Parkinson disease protein 2, PDJ, PRKN

Background

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin–protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson’s disease. It has 8 isoforms produced by alternative splicing.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

PARK2/Parkin Rabbit Polyclonal antibody

Elyssa (Verified Customer), 11-11-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
T47D, MCF7, CAMA1 (only worked in T47D)
Lot Number
This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.

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