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PARK2/Parkin Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 14060-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

PARK2/Parkin Antibody WB mouse brain tissue 14060-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14060-1-AP( PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

PARK2/Parkin Antibody IHC mouse brain tissue 14060-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PARK2/Parkin Antibody IHC mouse brain tissue 14060-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 14060-1-AP( PARK2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PARK2/Parkin Antibody IF SH-SY5Y cells 14060-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 14060-1-AP(PARK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, C6 cells, PC-12 cells, SH-SY5Y cells
Positive IHC detected in:
mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:100-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PARK2/Parkin fusion protein Ag5092
Full name:
Parkinson disease (autosomal recessive, juvenile) 2, parkin
Calculated molecular weight:
52 kDa
Observed molecular weight:
52 kDa
GenBank accession number:
BC022014
Gene ID (NCBI):
5071
Gene symbol
PARK2
Synonyms
AR JP, LPRS2, PARK2, Parkin, Parkinson disease protein 2, PDJ, PRKN

Background

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin–protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson’s disease. It has 8 isoforms produced by alternative splicing.


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