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CD31 Antibody 40 Publications

Rabbit Polyclonal| Catalog number: 11265-1-AP

Featured Product KD/KO validated

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Con: 59 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

CD31 Antibody WB Jurkat cells 11265-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 11265-1-AP( CD31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CD31 Antibody IHC human hepatocirrhosis tissue 11265-1-AP

Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 10x lens).

CD31 Antibody IHC human hepatocirrhosis tissue 11265-1-AP

Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 40x lens).

CD31 Antibody IP Jurkat cells 11265-1-AP

IP Result of anti-PECAM1,CD31 (IP:11265-1-AP, 5ug; Detection:11265-1-AP 1:300) with Jurkat cells lysate 4000ug.

CD31 Antibody IF human tonsillitis tissue 11265-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed human tonsillitis tissue using 11265-1-AP(CD31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CD31 Antibody FC HUVEC cells 11265-1-AP

1X10^6 HUVEC cells were stained with 0.2ug CD31 antibody (11265-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, monkey
Positive WB detected in:
Jurkat cells
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human hepatocirrhosis tissue, human tonsillitis tissue, human kidney tissue, human renal cell carcinoma tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human tonsillitis tissue
Positive FC detected in:
HUVEC cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:800-1:4000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD31 fusion protein Ag1787
Full name:
platelet/endothelial cell adhesion molecule
Calculated molecular weight:
83 kDa
Observed molecular weight:
130 kDa
GenBank accession number:
BC022512
Gene ID (NCBI):
5175
Gene symbol
PECAM1
Synonyms
CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

Background

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein with a molecular mass of approximately 120-140 kDa. It is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).


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