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CD31 Antibody 29 Publications

Rabbit Polyclonal| Catalog number: 11265-1-AP

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Con: 59 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

CD31 Antibody WB Jurkat cells 11265-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 11265-1-AP( CD31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CD31 Antibody IHC human hepatocirrhosis tissue 11265-1-AP

Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 10x lens).

CD31 Antibody IHC human hepatocirrhosis tissue 11265-1-AP

Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 40x lens).

CD31 Antibody IP Jurkat cells 11265-1-AP

IP Result of anti-PECAM1,CD31 (IP:11265-1-AP, 5ug; Detection:11265-1-AP 1:300) with Jurkat cells lysate 4000ug.

CD31 Antibody IF human tonsillitis tissue 11265-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed human tonsillitis tissue using 11265-1-AP(CD31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CD31 Antibody FC HUVEC cells 11265-1-AP

1X10^6 HUVEC cells were stained with 0.2ug CD31 antibody (11265-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, monkey
Positive WB detected in:
Jurkat cells
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human hepatocirrhosis tissue, human tonsillitis tissue, human kidney tissue, human renal cell carcinoma tissue, human lung cancer tissue, human endometrial cancer tissue, human gliomas tissue, human hysteromyoma tissue, human liver tissue
Positive IF detected in:
human tonsillitis tissue
Positive FC detected in:
HUVEC cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:800-1:4000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD31 fusion protein Ag1787
Full name:
platelet/endothelial cell adhesion molecule
Calculated molecular weight:
83 kDa
Observed molecular weight:
130 kDa
GenBank accession number:
BC022512
Gene ID (NCBI):
5175
Gene symbol
PECAM1
Synonyms
CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

Background

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein with a molecular mass of approximately 120-140 kDa. It is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).


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