CoraLite®594-conjugated CD31 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD31 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-11265

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200, CoraLite®488 Cytokeratin 7 antibody (CL488-15539, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta).

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  IF Staining of human breast cancer using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200, CoraLite®488 Cytokeratin 7 antibody (CL488-15539, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta).

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  IF Staining of HUVEC using CL594-11265

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human breast cancer tissue, human tonsillitis tissue, HUVEC cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-11265 targets CD31 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD31 fusion protein Ag1787
• Full Name: platelet/endothelial cell adhesion molecule
• Calculated Molecular Weight: 83 kDa
• GenBank Accession Number: BC022512
• Gene Symbol: PECAM1
• Gene ID (NCBI): 5175
• RRID: AB_2919762
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
human	IF	Stem Cell Res Ther; Cell heterogeneity, rather than the cell storage solution, affects the behavior of mesenchymal stem cells in vitro and in vivo.; Authors - Yong-Hong Wang