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  • KD/KO Validated

PIN1 Polyclonal antibody

PIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10495-1-AP

Synonyms

DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10495-1-AP targets PIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIN1 fusion protein Ag0767
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC002899
Gene symbol PIN1
Gene ID (NCBI) 5300
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus.

Protocols

Product Specific Protocols
WB protocol for PIN1 antibody 10495-1-APDownload protocol
IHC protocol for PIN1 antibody 10495-1-APDownload protocol
IF protocol for PIN1 antibody 10495-1-APDownload protocol
IP protocol for PIN1 antibody 10495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

Deficiency of microRNA-628-5p promotes the progression of gastric cancer by upregulating PIN1.

Authors - Yang Chen
  • KD Validated
mouseWB,IF

Neurochem Res

Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model.

Authors - Lan Tang
humanWB,IF

Virus Res

The peptidyl-prolyl cis/trans isomerase Pin1 interacts with three early regulatory proteins of human cytomegalovirus.

Authors - Martin Schütz
  • KD Validated
mouseWB

Int J Mol Sci

The Essential Role of Pin1 via NF-κB Signaling in Vascular Inflammation and Atherosclerosis in ApoE(-/-) Mice.

Authors - Ming Liu
  • KD Validated
mouseIF

J Cell Physiol

TGF-β1-PML SUMOylation-peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) form a positive feedback loop to regulate cardiac fibrosis.

Authors - Di Wu
humanWB

Int J Biol Sci

Cellular glucose metabolism is essential for the reduction of cell-impermeable water-soluble tetrazolium (WST) dyes.

Authors - Linna Xie