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PIN1 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 10495-1-AP

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

PIN1 Antibody WB HEK-293 cells 10495-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP(PIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

PIN1 Antibody IP HepG2 cells 10495-1-AP

IP Result of anti-PIN1 (IP:10495-1-AP, 4ug; Detection:10495-1-AP 1:500) with HepG2 cells lysate 2400ug.

PIN1 Antibody IHC human renal cell carcinoma tissue 10495-1-AP

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP( PIN1 Antibody) at dilution of 1:50 (under 10x lens)

PIN1 Antibody IHC human renal cell carcinoma tissue 10495-1-AP

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP( PIN1 Antibody) at dilution of 1:50 (under 40x lens)

PIN1 Antibody IF HEK-293 cells 10495-1-AP

Immunofluorescent analysis of HEK-293 cells using 10495-1-AP(PIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, HeLa cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human renal cell carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HEK-293 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PIN1 fusion protein Ag0767
Full name:
peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Calculated molecular weight:
18 kDa
Observed molecular weight:
18 kDa
GenBank accession number:
BC002899
Gene ID (NCBI):
5300
Gene symbol
PIN1
Synonyms
DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5

Background

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus.


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