- Featured Product
- KD/KO Validated
PINK1 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 23274-1-AP
BRPK, PARK6, PINK1, PTEN induced putative kinase 1
|Positive WB detected in||HeLa cells, mouse liver tissue, Jurkat cells|
|Positive IHC detected in||mouse brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 23274-1-AP immunogen is Fusion Protein PINK1 protein expressed in E. coli.
|Cited Species||human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PINK1 fusion protein Ag19825|
|Full Name||PTEN induced putative kinase 1|
|Calculated molecular weight||581aa,63 kDa|
|Observed molecular weight||65 kDa, 45 kDa|
|GenBank accession number||BC028215|
|Gene ID (NCBI)||65018|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa fomrs (PMID: 18221368; 25108683; 18031932). The half life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.
Ecotoxicol Environ Saf
Bone marrow mesenchymal stem cells repair Cr (VI)- injured kidney by regulating mitochondria-mediated apoptosis and mitophagy mediated via the MAPK signaling pathway.
Front Endocrinol (Lausanne)
Hepatic DNA Damage Induced by Electronic Cigarette Exposure Is Associated With the Modulation of NAD+/PARP1/SIRT1 Axis.
Oxid Med Cell Longev
Bcl-2 Proteins Regulate Mitophagy in Lipopolysaccharide-Induced Acute Lung Injury via PINK1/Parkin Signaling Pathway.
BMC Complement Med Ther
Qiliqiangxin reduced cardiomyocytes apotosis and improved heart function in infarcted heart through Pink1/Parkin -mediated mitochondrial autophagy.
Biochem Biophys Res Commun
FOXO3 protects nucleus pulposus cells against apoptosis under nutrient deficiency via autophagy.
Treadmill Exercise Attenuates Aβ-Induced Mitochondrial Dysfunction and Enhances Mitophagy Activity in APP/PS1 Transgenic Mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.
HONGXUE (Verified Customer) (08-19-2019)
The specific is not good. You can detect many bands using WB.