Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-12 cells, CCCP treated HEK-293 cells, CCCP treated HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23274-1-AP targets PINK1 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, monkey, bovine, goat, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19825 Product name: Recombinant human PINK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-581 aa of BC028215 Sequence: PRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL Predict reactive species |
| Full Name | PTEN induced putative kinase 1 |
| Calculated Molecular Weight | 581 aa, 63 kDa |
| Observed Molecular Weight | 65 kDa, 45 kDa |
| GenBank Accession Number | BC028215 |
| Gene Symbol | PINK1 |
| Gene ID (NCBI) | 65018 |
| RRID | AB_2879244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9BXM7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PINK1 antibody 23274-1-AP | Download protocol |
| IHC protocol for PINK1 antibody 23274-1-AP | Download protocol |
| WB protocol for PINK1 antibody 23274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PINK1 antibody (Cat# 23274-1-AP) has 772 citations published across 50+ journals, including Autophagy, Nature Cell Biology, Nature Communications, Cell Death & Disease, Phytomedicine, Redox Biology, and Molecular Cell. Research fields span mitophagy, mitochondrial quality control, neurodegeneration (Alzheimer's, Parkinson's), cancer, cardiovascular disease, diabetes, osteoarthritis, fibrosis, and metabolic disorders, with Western blot being the most frequently used application.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics | ||
Cell Metab Excessive vigorous exercise impairs cognitive function through a muscle-derived mitochondrial pretender. | ||
Bioact Mater NIR-responsive bio-system with sequential antibacterial and immunomodulatory effects for the treatment of periodontitis | ||
Bioact Mater Copper doped bioactive glass promotes matrix vesicles-mediated biomineralization via osteoblast mitophagy and mitochondrial dynamics during bone regeneration | ||
Bioact Mater Astaxanthin-metformin conjugate nanomicelles-loaded hydrogel reprograms macrophage metabolism via mitochondrion-targeted regulation for diabetic wound healing. | ||
Bioact Mater Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy. |
Reviews
What customers say
Most reviewers used this PINK1 antibody for Western blot at 1:1000 dilution, with generally positive results in mouse tissues and HEK cells. Several users confirmed clear PINK1 band detection, though a few noted weak non-specific bands. One reviewer reported poor specificity with multiple bands in HepG2 lysate. One negative review described failure to detect the expected 63 kDa band and no signal in immunofluorescence. Overall, the antibody performs well for WB in mouse samples, but results may vary by cell type and tissue, and optimization of dilution and blocking conditions is recommended.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rhiannon (Verified Customer) (09-16-2026) | No band at 63 kDa in CCCP or DMSO treated cells on western blot with TBS-T + 5% BSA. Band at 45 kDa as shown on product images however no reduction in this band upon CCCP treatment and no band showing 55 kDa major cleavage product so very uncertain that bands shown are due to specific PINK1 antibody binding. Antibody also appears to bind 70 kDa and 100 kDa marker (although not a problem). No signal at all with IF using suggested protocol from Proteintech.
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MALLIKARJUNA (Verified Customer) (10-17-2025) | GOOD QUALITY AB FOR WB
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Javier (Verified Customer) (09-09-2025) | Very good for Western Blotting.
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aidan (Verified Customer) (04-10-2024) | Good results. Would try higher dilution next time (1:500) on precast gel. Used handcast gel overnight incubation at 4 degrees. Blocked 1 hr 5% milk
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Nin (Verified Customer) (02-27-2023) | It is okay to probe PINK1 with two bands although there are some weak non-specific bands.
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Yahir Alberto (Verified Customer) (11-12-2021) | The antibody works fine with overnight incubation at 4 °C.
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Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
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HONGXUE (Verified Customer) (08-19-2019) | The specific is not good. You can detect many bands using WB.
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