iPLA2 Polyclonal antibody
iPLA2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22030-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human small intestine tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
22030-1-AP targets iPLA2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | iPLA2 fusion protein Ag16896 |
| Full Name | phospholipase A2, group VI (cytosolic, calcium-independent) |
| Calculated Molecular Weight | 90 kDa |
| Observed Molecular Weight | 80 kDa, 88 kDa |
| GenBank Accession Number | BC036742 |
| Gene Symbol | PLA2G6 |
| Gene ID (NCBI) | 8398 |
| RRID | AB_2878976 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLA2G6, also named as PLPLA9, is involved in the remodeling of membranes allowing arachidonic acid to be placed in the proper position in phospholipids for stimulated release. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. This protein has 4 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for iPLA2 antibody 22030-1-AP | Download protocol |
| IHC protocol for iPLA2 antibody 22030-1-AP | Download protocol |
| IP protocol for iPLA2 antibody 22030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition | ||
J Agric Food Chem Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism. | ||
Neurol Res Calcium-independent phospholipase A2 inhibitor produces an analgesic effect in a rat model of neuropathic pain by reducing central sensitization in the dorsal horn. | ||
Zhongguo Dang Dai Er Ke Za Zhi [Clinical features of infantile neuroaxonal dystrophy and PLA2G6 gene testing]. | ||
Theranostics Ferritin light chain promotes the reprogramming of glioma immune microenvironment and facilitates glioma progression | ||
Molecules Identification of Diagnostic Metabolic Signatures in Thyroid Tumors Using Mass Spectrometry Imaging |
