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PPA1 Polyclonal antibody

PPA1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14985-1-AP

Synonyms

Inorganic pyrophosphatase, IOPPP, PP, PP1, PPA1, PPase, pyrophosphatase (inorganic) 1, SID6 8061

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using PPA1 Polyclonal antibody (14985-1-AP)

    WB analysis of human heart using 14985-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PPA1 Polyclonal antibody (14985-1-AP)

    WB analysis of human liver using 14985-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PPA1 Polyclonal antibody (14985-1-AP)

    WB analysis of human liver using 14985-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using PPA1 Polyclonal antibody (14985-1-AP)

    IP experiment of mouse liver using 14985-1-AP

    IP result of anti-PPA1 (IP:14985-1-AP, 4ug; Detection:14985-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPA1 Polyclonal antibody (14985-1-AP)

    IHC staining of human breast cancer using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPA1 Polyclonal antibody (14985-1-AP)

    IHC staining of human breast cancer using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using PPA1 Polyclonal antibody (14985-1-AP)

    IHC staining of human heart using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PPA1 Polyclonal antibody (14985-1-AP)

    IHC staining of human heart using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPA1 Polyclonal antibody (14985-1-AP)

    IF Staining of HeLa using 14985-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14985-1-AP (PPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman heart tissue, human liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14985-1-AP targets PPA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPA1 fusion protein Ag6969
Full Name pyrophosphatase (inorganic) 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001022
Gene Symbol PPA1
Gene ID (NCBI) 5464
RRIDAB_2167890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

Protocols

Product Specific Protocols
WB protocol for PPA1 antibody 14985-1-APDownload protocol
IHC protocol for PPA1 antibody 14985-1-APDownload protocol
IF protocol for PPA1 antibody 14985-1-APDownload protocol
IP protocol for PPA1 antibody 14985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Metabolites

PPA1 Deficiency Causes a Deranged Galactose Metabolism Recognizable in Neonatal Screening

Authors - Melanie T Achleitner
View Article
humanWB

Sci Rep

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Authors - Hexiao Shen
View Article