|Positive WB detected in||HepG2 cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat liver tissue, HuH-7 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11067-2-AP targets PROX1 in WB, IP, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PROX1 fusion protein Ag1543|
|Full Name||prospero homeobox 1|
|Calculated molecular weight||737 aa, 83 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC024201|
|Gene ID (NCBI)||5629|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Prospero-related homeobox 1 (Prox1) is a homeobox transcription factor involved in various developmental processes, including neurogenesis and lymphangiogenesis. In the central nervous system (CNS), it controls the maintenance, proliferation, and differentiation of neural stem cells. This is a rabbit polyclonal antibody raised against part of C-terminal chain of human PROX1. The calcualted molecular weight of PROX1 is 83 kDa.
What is the molecular weight of KEAP1 protein? Is Prox1 post-translationally modified?
The calculated molecular weight of Prox1 protein is 83 kDa but the observed weight is 95 kDa. Prox1 is a subject of SUMO-ylation at K556, which is important for its transcription activity and increases the molecular weight of Prox1 to 110 kDa (PMID: 19706680). Usually there is a pool of modified and unmodified Prox1 protein in cells and therefore it is quite common to observe two specific bands in western blotting using Prox1 antibody.
What is the subcellular localization of Prox1?
Prox1 contains a nuclear localization signal within its N-terminus and resides in the nucleus, where it acts as a transcription factor.
What is the tissue expression pattern of Prox1 in the central nervous system?
Prox1 expression in the central nervous system (CNS) depends on the developmental stage. During embryogenesis Prox1 is present in neuronal precursors located in the subventricular zone, while during fetal development it is additionally present in the cerebral and cerebellar cortex and hippocampus. Postnatally, Prox1 expression in the CNS is restricted to the dentate gyrus of the hippocampus and cerebellum (PMID: 17117441). Therefore, Prox1 can be used as a marker of a subset of neuronal progenitors, as well as a specific marker of dentate gyrus cell lineage, such as type-2 intermediate progenitors and mature granule cells.
Is Prox1 expressed outside of the central nervous system?
Yes. Prox1 is expressed in the developmental stages of the eye lens, liver, pancreas, heart, and the lymphatic system (PMID: 22733308). Prox1 is also expressed post-developmentally in lymphatic endothelial cells and is often used, together with VEGFR-3, as a marker for lymphatic vessels.
Meningeal lymphatic vessels at the skull base drain cerebrospinal fluid.
The role of fatty acid β-oxidation in lymphangiogenesis.
Neurotensin is an anti-thermogenic peptide produced by lymphatic endothelial cells.
Prospero-related homeobox 1 drives angiogenesis of hepatocellular carcinoma through selectively activating interleukin-8 expression.
The Prox1-Vegfr3 feedback loop maintains the identity and the number of lymphatic endothelial cell progenitors.
Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guang (Verified Customer) (11-12-2018)
We use heat antigen retrieval , but we didn't get any result by using this antibody. If it is possible, you can send me another free sample of this type. I will test it by using the enzymatic antigen retrieval or EDTA method to perform the Immunofluorescence.