Gamma Catenin Monoclonal antibody

Gamma Catenin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, dog

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A5D10

Cat no : 66445-1-Ig

Synonyms

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin



Tested Applications

Positive WB detected inhuman heart tissue, A431 cells, HT-29 cells, MDCK cells, rat heart tissue, T-47D cells
Positive IHC detected inhuman breast cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66445-1-Ig targets Gamma Catenin in WB, IHC, ELISA applications and shows reactivity with human, rat, pig, dog samples.

Tested Reactivity human, rat, pig, dog
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Gamma Catenin fusion protein Ag1627
Full Name junction plakoglobin
Calculated molecular weight 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC011865
Gene symbol JUP
Gene ID (NCBI) 3728
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Protocols

Product Specific Protocols
WB protocol for Gamma Catenin antibody 66445-1-IgDownload protocol
IHC protocol for Gamma Catenin antibody 66445-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols