CoraLite® Plus 488-conjugated Gamma Catenin Monoclonal antibody

Gamma Catenin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, Canine

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5D10

Cat no : CL488-66445

Synonyms

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

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Validation Data Gallery

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  WB analysis using CL488-66445

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL488-66445

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HT-29 cells, T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66445 targets Gamma Catenin in WB applications and shows reactivity with human, rat, pig, Canine samples.

• Tested Reactivity: human, rat, pig, Canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Gamma Catenin fusion protein Ag1627
• Full Name: junction plakoglobin
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC011865
• Gene Symbol: JUP
• Gene ID (NCBI): 3728
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.