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  • KD/KO Validated

RAB11A/B Polyclonal antibody

RAB11A/B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15903-1-AP

Synonyms

Rab 11, RAB11, RAB11A, Ras related protein Rab 11A, YL8



Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HeLa cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15903-1-AP targets RAB11A/B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity canine, human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11A/B fusion protein Ag8742
Full Name RAB11A, member RAS oncogene family
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC013348
Gene symbol RAB11A
Gene ID (NCBI) 8766
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

Protocols

Product Specific Protocols
WB protocol for RAB11A/B antibody 15903-1-APDownload protocol
IHC protocol for RAB11A/B antibody 15903-1-APDownload protocol
IF protocol for RAB11A/B antibody 15903-1-APDownload protocol
IP protocol for RAB11A/B antibody 15903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyIF

Cell Commun Signal

Zika virus: mapping and reprogramming the entry.

Authors - Katarzyna Owczarek
humanWB,IHC

Med Sci Monit

Rab11 Functions as an Oncoprotein via Nuclear Factor kappa B (NF-κB) Signaling Pathway in Human Bladder Carcinoma.

Authors - Xue Gong
humanWB,IHC

J Oncol

Rab11a Is Overexpressed in Gastric Cancer and Regulates FAK/AKT Signaling.

Authors - Jiang Du
humanIHC

Dig Dis Sci

Inactivation of MYO5B promotes invasion and motility in gastric cancer cells.

Authors - Dong Wenjie W
humanWB

Tumour Biol

AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.

Authors - Zhaoyang Zeng
humanIF

Vet Res

Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells.

Authors - Artur Szczepanski

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Stephen (Verified Customer) (09-12-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours rab11 antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: mouse OC-2 cells
RAB11A/B Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC-2 cells (Cat no:15903-1-AP)