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Rab18 Polyclonal antibody

Rab18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11304-1-AP

Synonyms

RAB18, RAB18LI1, Ras related protein Rab 18



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, human brain tissue, PC-3 cells, human testis tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11304-1-AP targets Rab18 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rab18 fusion protein Ag1841
Full Name RAB18, member RAS oncogene family
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC015014
Gene Symbol RAB18
Gene ID (NCBI) 22931
RRIDAB_2173932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB18 is a member of a family of Ras-related small GTPases. RAB18 plays a role in apical endocytosis/recycling, and may be implicated in transport between the plasma membrane and early endosomes. It also plays a key role in eye and brain development and neurodegeneration. Defects in RAB18 are associated with Warburg micro syndrome type 3.

Protocols

Product Specific Protocols
WB protocol for Rab18 antibody 11304-1-APDownload protocol
IHC protocol for Rab18 antibody 11304-1-APDownload protocol
IF protocol for Rab18 antibody 11304-1-APDownload protocol
IP protocol for Rab18 antibody 11304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Clin Invest

RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia

Authors - Nathalie Launay
monkeyWB

J Cell Biol

Rab18 and a Rab18 GEF complex are required for normal ER structure.

Authors - Andreas Gerondopoulos
mouseIF

Dev Cell

KIF3B promotes a PI3K signaling gradient causing changes in a Shh protein gradient and suppressing polydactyly in mice

Authors - Shuo Wang
humanWB

PLoS Pathog

Rab18 Binds to Hepatitis C Virus NS5A and Promotes Interaction between Sites of Viral Replication and Lipid Droplets.

Authors - Salloum Shadi S
mouseWB

Food Chem

Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.

Authors - Shanshan Hu
humanWB,IHC

Carcinogenesis

Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells.

Authors - You Xiaona X
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