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ADRP/Perilipin 2 Polyclonal antibody
ADRP/Perilipin 2 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 15294-1-AP
Validation Data Gallery
|Positive WB detected in||mouse liver tissue, K-562 cells, rat liver tissue, NIH/3T3 cells|
|Positive IHC detected in||human liver cancer tissue, mouse liver tissue, human liver tissue, human renal cell carcinoma tissue, human prostate cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||oleic acid treated HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:8000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
15294-1-AP targets ADRP/Perilipin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey, zebrafish, pig, canine, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ADRP/Perilipin 2 fusion protein Ag7539|
|Full Name||adipose differentiation-related protein|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||45-48 kDa|
|GenBank accession number||BC005127|
|Gene ID (NCBI)||123|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.
|Product Specific Protocols|
|WB protocol for ADRP/Perilipin 2 antibody 15294-1-AP||Download protocol|
|IHC protocol for ADRP/Perilipin 2 antibody 15294-1-AP||Download protocol|
|IF protocol for ADRP/Perilipin 2 antibody 15294-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.
Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.
Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy
Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.
O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.
Cell Death Differ
Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christine (Verified Customer) (09-19-2023)
Tried on HEK293T cells treated with 300 uM oleic acid overnight to induce the formation of lipid droplets. This antibody decorates some droplets but was a bit weak. TIP47 antibody (10694-1-AP) performed better (clearer detection of the droplets) at the same concentration (1:100).
Kamal (Verified Customer) (07-05-2023)
Worked well when diluted with 1X PBS.
Christin (Verified Customer) (03-09-2023)
Great antibody for WB with low background signal.
Nick (Verified Customer) (04-20-2022)
Helped localize expression of Plin2
Boyan (Verified Customer) (05-09-2019)
Very good for WB