Perilipin-2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Perilipin-2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

143

Reviews (7)

Cat no : 15294-1-AP

Synonyms

ADFP, Perilipin 2, ADRP/Perilipin 2, ADRP, Adipose differentiation-related protein

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Validation Data Gallery

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  WB analysis using 15294-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15294-1-AP (ADRP/Perilipin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15294-1-AP (ADRP/Perilipin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin-2 antibody (15294-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 15294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, K-562 cells, rat liver tissue, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human renal cell carcinoma tissue, human liver tissue, human colon cancer tissue, human prostate cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: oleic acid treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 87 publications below
• IHC: See 24 publications below
• IF: See 55 publications below

Product Information

15294-1-AP targets Perilipin-2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, monkey, zebrafish, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Perilipin-2 fusion protein Ag7539
• Full Name: adipose differentiation-related protein
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC005127
• Gene Symbol: Perilipin-2
• Gene ID (NCBI): 123
• RRID: AB_2878122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Publications

Species	Application	Title
human	IF	Nature; iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer; Authors - Dong Shin Park
human	IF	Nature; The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.; Authors - Simona Ricciardi
mouse,human	IF,IHC	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	IF	Nat Commun; Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy; Authors - Donghai Cui
mouse	WB	Brain Behav Immun; HIV-TAT dysregulates microglial lipid metabolism through SREBP2/miR-124 axis: Implication of lipid droplet accumulation microglia in NeuroHIV; Authors - Yan Cheng
mouse	WB	Mol Cell; Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.; Authors - Anyuan He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christin (Verified Customer) (11-25-2024)

Excellent antibody for ICC and WB of human iPSC-derived microglia and macrophages

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human iPSC-derived microglia and macrophages

FH

Liliana (Verified Customer) (02-13-2024)

WORKS VERY WELL ON PH9, 1:2000 AND ON PH6, 1:1000

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: SKIN WITH SEBACCEUS ADENOMA

Perilipin-2 Antibody Immunohistochemistry validation (1:2000 dilution) in SKIN WITH SEBACCEUS ADENOMA (Cat no:15294-1-AP)

FH

Christine (Verified Customer) (09-19-2023)

Tried on HEK293T cells treated with 300 uM oleic acid overnight to induce the formation of lipid droplets. This antibody decorates some droplets but was a bit weak. TIP47 antibody (10694-1-AP) performed better (clearer detection of the droplets) at the same concentration (1:100).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293T

FH

Kamal (Verified Customer) (07-05-2023)

Worked well when diluted with 1X PBS.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Liver tissue

FH

Christin (Verified Customer) (03-09-2023)

Great antibody for WB with low background signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human iPSC-derived microglia and macrophages

FH

Nick (Verified Customer) (04-20-2022)

Helped localize expression of Plin2

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Cell Tissue Type: 3T3-L1

FH

Boyan (Verified Customer) (05-09-2019)

Very good for WB

• Applications: Western Blot,
• Cell Tissue Type: MEF

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