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ADRP/Perilipin 2 Polyclonal antibody

ADRP/Perilipin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15294-1-AP

Synonyms

ADFP, Adipophilin, ADRP, Perilipin 2, PLIN2



Tested Applications

Positive WB detected in3T3-L1 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human colon cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inoleic acid treated HepG2 cells, oleic acid treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15294-1-AP targets ADRP/Perilipin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRP/Perilipin 2 fusion protein Ag7539
Full Name adipose differentiation-related protein
Calculated molecular weight 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC005127
Gene symbol ADFP
Gene ID (NCBI) 123
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Protocols

Product Specific Protocols
WB protocol for ADRP/Perilipin 2 antibody 15294-1-APDownload protocol
IHC protocol for ADRP/Perilipin 2 antibody 15294-1-APDownload protocol
IF protocol for ADRP/Perilipin 2 antibody 15294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Nutr Food Res

Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis in vivo and vitro.

Authors - Peng Lei
mouseWB

Diabetes

PMID: 30617219

Authors - Stacey N Keenan
humanIF

Proc Natl Acad Sci U S A

Recruitment of APOL1 kidney disease risk variants to lipid droplets attenuates cell toxicity.

Authors - Justin Chun
mouseWB

Mol Cell Biol

Cell cycle progression regulates biogenesis and cellular localization of lipid droplets.

Authors - André L S Cruz
human,monkeyWB

Cell Rep

The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation.

Authors - Dongfang Li
humanWB,IHC,IF

Metabolism

Impaired adipogenic capacity in induced pluripotent stem cells from lipodystrophic patients with BSCL2 mutations.

Authors - Eisaku Mori

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (05-09-2019)

Very good for WB

  • Applications: Western Blot,
  • Cell Tissue Type: MEF