KLC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

KLC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 19028-1-AP

Synonyms

KNS2A, KNS2, KLC 1, KLC

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Validation Data Gallery

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  WB analysis of mouse brain using 19028-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 19028-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19028-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19028-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19028-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 19028-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human bowen disease using 19028-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19028-1-AP (KLC1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19028-1-AP

  Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 19028-1-AP

  Immunofluorescent analysis of HepG2 cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 19028-1-AP

  Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, HepG2 cells, SH-SY5Y cells
• Positive IHC detected in: human brain tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:100-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

19028-1-AP targets KLC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLC1 fusion protein Ag13570
• Full Name: kinesin light chain 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 61-70 kDa
• GenBank Accession Number: BC008881
• Gene Symbol: KLC1
• Gene ID (NCBI): 3831
• RRID: AB_10641041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	FASEB J; Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.; Authors - Amélie Robert ; KO Validated
human	WB,IF	Dev Neurobiol; Forebrain commissure formation in zebrafish embryo requires the binding of KLC1 to CRMP2; Authors - Simo Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognised the expected bands just like what is shown in its technical paper.

• Applications: Western Blot