KLC1 Polyclonal antibody

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  WB analysis using 19028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19028-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 19028-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 19028-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19028-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 19028-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 19028-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human bowen disease using 19028-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19028-1-AP (KLC1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19028-1-AP

  Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 19028-1-AP

  Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 19028-1-AP

  Immunofluorescent analysis of HepG2 cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• Featured Product
• KD/KO Validated

KLC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19028-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KLC, KLC 1, KNS2, KNS2A

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, COLO 320 cells, HepG2 cells, mouse brain tissue, SK-N-SH cells
• Positive IHC detected in: human brain tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:100-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

19028-1-AP targets KLC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLC1 fusion protein Ag13570
• Full Name: kinesin light chain 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 61-70 kDa
• GenBank Accession Number: BC008881
• Gene Symbol: KLC1
• Gene ID (NCBI): 3831
• RRID: AB_10641041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q07866
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Kinesin is tetrameric proteins containing two heavy (alpha) chains and two light (beta) chains. The alpha chains provide the tubulin binding site and the ATPase domains, whereas the beta chains are responsible for the specific attachment of the organelle to be moved by the kinesin tetramer (PubMed:21385839).

Publications

Species	Application	Title
human	WB	FASEB J; Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.; Authors - Amélie Robert ; KO Validated
human	WB,IF	Dev Neurobiol; Forebrain commissure formation in zebrafish embryo requires the binding of KLC1 to CRMP2; Authors - Simo Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognised the expected bands just like what is shown in its technical paper.

• Applications: Western Blot