H2AFY Polyclonal antibody

H2AFY Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26875-1-AP

Synonyms

Core histone macro H2A.1, H2A histone family, member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2AFY, Histone H2A.y, Histone macroH2A1, MACROH2A1, MACROH2A1.1, macroH2A1.2, mH2A1

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Validation Data Gallery

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  WB analysis using 26875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26875-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 26875-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26875-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26875-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 26875-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using H2AFY antibody (26875-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, Caco-2 cells, mouse brain tissue, HeLa cells, rat brain
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 26875-1-AP is H2AFY Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: H2AFY fusion protein Ag25445
• Full Name: H2A histone family, member Y
• Calculated Molecular Weight: 372 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC013331
• Gene Symbol: H2AFY
• Gene ID (NCBI): 9555
• RRID: AB_2918113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Cell Death Discov; CYB561 promotes HER2+ breast cancer proliferation by inhibiting H2AFY degradation; Authors - Ting Zhao