RAN Polyclonal antibody

RAN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10469-1-AP

Synonyms

ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4



Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, rat kidney tissue, NIH/3T3 cells, mouse kidney tissue, HeLa cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10469-1-AP targets RAN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, M. rosenbergii, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAN fusion protein Ag0748
Full Name RAN, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC004272
Gene symbol RAN
Gene ID (NCBI) 5901
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAN antibody 10469-1-APDownload protocol
IHC protocol for RAN antibody 10469-1-APDownload protocol
IF protocol for RAN antibody 10469-1-APDownload protocol
IP protocol for RAN antibody 10469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochim Biophys Acta Mol Cell Res

SIRT7 regulates the nuclear export of NF-κB p65 by deacetylating Ran.

Authors - Shihab U Sobuz
M. rosenbergiiWB

Fish Shellfish Immunol

Identification and function analysis of ras-related nuclear protein from Macrobrachium rosenbergii involved in Spiroplasma eriocheiris infection.

Authors - Mingxiao Ning
humanWB

Int J Biol Sci

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Authors - Yancheng Zhong
  • KD Validated
humanIHC

Oncotarget

Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.

Authors - Zhen Chen
ratIP,WB

Cell Commun Signal

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Authors - Abiola Abdulrahman Ayanlaja