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  • Featured Product
  • KD/KO Validated

RBBP6 Polyclonal antibody

RBBP6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11882-1-AP

Synonyms

MY038, P2P R, P2PR, PACT, Protein P2P R, RBBP6, RBQ 1, RBQ1, SNAMA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBBP6 Polyclonal antibody (11882-1-AP)

    WB analysis using 11882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RBBP6 Polyclonal antibody (11882-1-AP)

    WB analysis using 11882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11882-1-AP targets RBBP6 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBBP6 fusion protein Ag2484
Full Name retinoblastoma binding protein 6
Calculated Molecular Weight 1792 aa, 200 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC029352
Gene Symbol RBBP6
Gene ID (NCBI) 5930
RRIDAB_2177794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

Protocols

Product Specific Protocols
WB protocol for RBBP6 antibody 11882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells

Authors - Christopher Noyes
  • KD Validated
View Article