Tested Applications
| Positive WB detected in | HL-60 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
14027-1-AP targets ROD1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5172 Product name: Recombinant human ROD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC044585 Sequence: MDASPSPFSLPKKLNELSARRGSDELLSSGIINGPFTMNSSTPSTANGNDSKKFKRDRPPCSPSRVLHLRKIPCDVTEAEIISLGLPFGKVTNLLMLKGKSQAFLEMASEEAAVTMVNYYTPITPHLRSQPVYIQYSNHRELKTDNLPNQARAQAALQAVSAVQSGSLALSGGPSNEGTVLPGQSPVLRIIIENLFYPVTLEVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVNAHYAKMALDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDFTRLDLPTGDGQPSLEPPMAAAFGAPGIISSPYAGAAGFAPAIGFPQATGLSVPAVPGALGPLTITSSAVTG Predict reactive species |
| Full Name | ROD1 regulator of differentiation 1 (S. pombe) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55-59 kDa |
| GenBank Accession Number | BC044585 |
| Gene Symbol | ROD1 |
| Gene ID (NCBI) | 9991 |
| RRID | AB_2182321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95758 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ROD1 is a RNA-binding protein that mediates pre-mRNA alternative splicing regulation, it plays a role in the regulation of cell proliferation, differentiation and migration. ROD1 exist some isoforms and also can be modified with the molecular weight range from 55-59 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ROD1 antibody 14027-1-AP | Download protocol |
| WB protocol for ROD1 antibody 14027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.
| ||
iScience Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis | ||
Cancer Cell Int PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression. | ||
Cancer Cell Int PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway. | ||
Oncol Lett Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus. | ||
Adv Sci (Weinh) Targeting PTBP3-Mediated Alternative Splicing of COX11 Induces Cuproptosis for Inhibiting Gastric Cancer Peritoneal Metastasis
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Yaroslav (Verified Customer) (08-03-2022) | multiple bands detected at all tested dulitions (1:3000-1:10000)
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