RTN4/NOGO Polyclonal antibody

RTN4/NOGO Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10740-1-AP

Synonyms

ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, SP1507



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10740-1-AP targets RTN4/NOGO in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTN4/NOGO fusion protein Ag1164
Full Name reticulon 4
Calculated molecular weight 130 kDa
Observed molecular weight 45 kDa, 190-210 kDa
GenBank accession numberBC007109
Gene symbol RTN4
Gene ID (NCBI) 57142
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.

Protocols

Product Specific Protocols
WB protocol for RTN4/NOGO antibody 10740-1-APDownload protocol
IHC protocol for RTN4/NOGO antibody 10740-1-APDownload protocol
FC protocol for RTN4/NOGO antibody 10740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Ther Med

miR-124 participates in the proliferation and differentiation of brain glioma stem cells through regulating Nogo/NgR expression.

Authors - Yun Meng
mouseWB

J Biol Chem

Sequestosome-1/p62 is Associated with Autophagic Removal of Excess Hepatic Endoplasmic Reticulum in Mice.

Authors - Hua Yang
humanWB

Autophagy

DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.

Authors - Xiaoyu Tian
human,mouseWB,IF

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng