RTN4/NOGO Polyclonal antibody

RTN4/NOGO Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10740-1-AP


ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, SP1507

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10740-1-AP targets RTN4/NOGO in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTN4/NOGO fusion protein Ag1164
Full Name reticulon 4
Calculated molecular weight 130 kDa
Observed molecular weight190-210 kDa, 45 kDa
GenBank accession numberBC007109
Gene symbol RTN4
Gene ID (NCBI) 57142
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.


Product Specific Protocols
WB protocol for RTN4/NOGO antibody 10740-1-APDownload protocol
IHC protocol for RTN4/NOGO antibody 10740-1-APDownload protocol
FC protocol for RTN4/NOGO antibody 10740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Fetal Pediatr Pathol

The Effect of Mild Hypothermia on Nogo-A and Neurological Function in the Brain after Cardiopulmonary Resuscitation in Rats.

Authors - Jia-Li Li

Exp Ther Med

miR-124 participates in the proliferation and differentiation of brain glioma stem cells through regulating Nogo/NgR expression.

Authors - Yun Meng

J Biol Chem

Sequestosome-1/p62 is Associated with Autophagic Removal of Excess Hepatic Endoplasmic Reticulum in Mice.

Authors - Hua Yang


DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.

Authors - Xiaoyu Tian

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang