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RTN4/NOGO Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10740-1-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

RTN4/NOGO Antibody WB mouse brain tissue 10740-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10740-1-AP(RTN4/NOGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

RTN4/NOGO Antibody IHC human prostate cancer tissue 10740-1-AP

Immunohistochemical of paraffin-embedded human prostate cancer using 10740-1-AP(RTN4/NOGO antibody) at dilution of 1:100 (under 10x lens)

RTN4/NOGO Antibody FC SH-SY5Y cells 10740-1-AP

1X10^6 SH-SY5Y cells were stained with 0.2ug RTN4/NOGO antibody (10740-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse
Positive WB detected in:
mouse brain tissue
Positive IHC detected in:
human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RTN4/NOGO fusion protein Ag1164
Full name:
reticulon 4
Calculated molecular weight:
130 kDa
Observed molecular weight:
45 kDa, 190-210 kDa
GenBank accession number:
BC007109
Gene ID (NCBI):
57142
Gene symbol
RTN4
Synonyms
ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, SP1507

Background

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.


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