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  • KD/KO Validated

RTN4/NOGO Polyclonal antibody

RTN4/NOGO Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10950-1-AP

Synonyms

ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, SP1507



Tested Applications

Positive WB detected inA375 cells, A549 cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10950-1-AP targets RTN4/NOGO in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTN4/NOGO fusion protein Ag1392
Full Name reticulon 4
Calculated molecular weight 220 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC012619
Gene symbol RTN4
Gene ID (NCBI) 57142
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.

Protocols

Product Specific Protocols
WB protocol for RTN4/NOGO antibody 10950-1-APDownload protocol
IHC protocol for RTN4/NOGO antibody 10950-1-APDownload protocol
IF protocol for RTN4/NOGO antibody 10950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Biol

Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.

Authors - Guang Gao
  • KD Validated
mouseWB

Neurol Res

Erythropoietin attenuates axonal injury after middle cerebral artery occlusion in mice.

Authors - Rongliang Wang
ratWB

Drug Des Devel Ther

Tanshinone IIA Promotes Axonal Regeneration in Rats with Focal Cerebral Ischemia Through the Inhibition of Nogo-A/NgR1/RhoA/ROCKII/MLC Signaling.

Authors - Jing Wang
humanWB,IF

Autophagy

Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.

Authors - Nicholas J Lennemann