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RTN4/NOGO Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10950-1-AP

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

RTN4/NOGO Antibody WB A375 cells 10950-1-AP

A375 cells were subjected to SDS PAGE followed by western blot with 10950-1-AP(RTN4/NOGO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

RTN4/NOGO Antibody IHC human testis tissue 10950-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 10950-1-AP( RTN4/NOGO antibody at dilution of 1:50 (under 10x lens)

RTN4/NOGO Antibody IHC human testis tissue 10950-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 10950-1-AP( RTN4/NOGO antibody at dilution of 1:50 (under 40x lens)

RTN4/NOGO Antibody IF A375 cells 10950-1-AP

Immunofluorescent analysis of A375 cells using 10950-1-AP(RTN4/NOGO antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
A375 cells, A549 cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected in:
human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A375 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RTN4/NOGO fusion protein Ag1392
Full name:
reticulon 4
Calculated molecular weight:
220 kDa
Observed molecular weight:
45-50 kDa
GenBank accession number:
BC012619
Gene ID (NCBI):
57142
Gene symbol
RTN4
Synonyms
ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, SP1507

Background

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.


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