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  • KD/KO Validated

RTN4/NOGO Polyclonal antibody

RTN4/NOGO Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10950-1-AP

Synonyms

ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, RTN4/NOGO, SP1507



Tested Applications

Positive WB detected inA375 cells, HepG2 cells, A549 cells, SH-SY5Y cells
Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10950-1-AP targets RTN4/NOGO in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTN4/NOGO fusion protein Ag1392
Full Name reticulon 4
Calculated Molecular Weight 130 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC012619
Gene Symbol RTN4
Gene ID (NCBI) 57142
RRIDAB_2877741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. Some isoforms of RTN4 have been described. RTN4A (Nogo-A, runs at ~200 kDa), RTN4B (Nogo-B, 40-55 kDa), and RTN4C (Nogo-C, 22-25 kDa) are three major isoforms (PMID: 31092426; 16469703).

Protocols

Product Specific Protocols
WB protocol for RTN4/NOGO antibody 10950-1-APDownload protocol
IHC protocol for RTN4/NOGO antibody 10950-1-APDownload protocol
IF protocol for RTN4/NOGO antibody 10950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Biol

Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.

Authors - Guang Gao
  • KD Validated
humanWB,IF

Autophagy

Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.

Authors - Nicholas J Lennemann
humanWB

J Cell Sci

SEC24A facilitates colocalization and calcium flux between endoplasmic reticulum and mitochondria.

Authors - Tamutenda Chidawanyika
ratWB

Drug Des Devel Ther

Tanshinone IIA Promotes Axonal Regeneration in Rats with Focal Cerebral Ischemia Through the Inhibition of Nogo-A/NgR1/RhoA/ROCKII/MLC Signaling.

Authors - Jing Wang
mouseWB

Neurol Res

Erythropoietin attenuates axonal injury after middle cerebral artery occlusion in mice.

Authors - Rongliang Wang
mouseWB

NPJ Parkinsons Dis

Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities

Authors - Jia Yu