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RTN4/NOGO Polyclonal antibody
RTN4/NOGO Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10950-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, A549 cells, HepG2 cells, SH-SY5Y cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A375 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IF | See 1 publications below |
Product Information
10950-1-AP targets RTN4/NOGO in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RTN4/NOGO fusion protein Ag1392 |
Full Name | reticulon 4 |
Calculated molecular weight | 220 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC012619 |
Gene symbol | RTN4 |
Gene ID (NCBI) | 57142 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.
Protocols
Product Specific Protocols | |
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WB protocol for RTN4/NOGO antibody 10950-1-AP | Download protocol |
IHC protocol for RTN4/NOGO antibody 10950-1-AP | Download protocol |
IF protocol for RTN4/NOGO antibody 10950-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
PLoS Biol Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.
| ||
Neurol Res Erythropoietin attenuates axonal injury after middle cerebral artery occlusion in mice. | ||
Drug Des Devel Ther Tanshinone IIA Promotes Axonal Regeneration in Rats with Focal Cerebral Ischemia Through the Inhibition of Nogo-A/NgR1/RhoA/ROCKII/MLC Signaling. | ||
Autophagy Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B. |