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- KD/KO Validated
RTN4/NOGO Polyclonal antibody
RTN4/NOGO Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10950-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, A549 cells, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
10950-1-AP targets RTN4/NOGO in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RTN4/NOGO fusion protein Ag1392 |
| Full Name | reticulon 4 |
| Calculated molecular weight | 220 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC012619 |
| Gene symbol | RTN4 |
| Gene ID (NCBI) | 57142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RTN4/NOGO antibody 10950-1-AP | Download protocol |
| IHC protocol for RTN4/NOGO antibody 10950-1-AP | Download protocol |
| IF protocol for RTN4/NOGO antibody 10950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Biol Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.
| ||
Neurol Res Erythropoietin attenuates axonal injury after middle cerebral artery occlusion in mice. | ||
Drug Des Devel Ther Tanshinone IIA Promotes Axonal Regeneration in Rats with Focal Cerebral Ischemia Through the Inhibition of Nogo-A/NgR1/RhoA/ROCKII/MLC Signaling. | ||
Autophagy Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B. |
