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SAP130 Polyclonal antibody

SAP130 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12130-1-AP

Synonyms

SAP130

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of HEK-293 using 12130-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of HepG2 using 12130-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of HeLa using 12130-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of Jurkat using 12130-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of K-562 using 12130-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SAP130 Polyclonal antibody (12130-1-AP)

    WB analysis of HeLa using 12130-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SAP130 Polyclonal antibody (12130-1-AP)

    IP experiment of HepG2 using 12130-1-AP

    IP result of anti-SAP130 (IP:12130-1-AP, 4ug; Detection:12130-1-AP 1:600) with HepG2 cells lysate 1000ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SAP130 Polyclonal antibody (12130-1-AP)

    IHC staining of human pancreas cancer using 12130-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12130-1-AP (SAP130 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SAP130 Polyclonal antibody (12130-1-AP)

    IF Staining of HepG2 using 12130-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12130-1-AP (SAP130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12130-1-AP targets SAP130 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAP130 fusion protein Ag2774
Full Name Sin3A-associated protein, 130kDa
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC017453
Gene Symbol SAP130
Gene ID (NCBI) 79595
RRIDAB_2186637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.

Protocols

Product Specific Protocols
WB protocol for SAP130 antibody 12130-1-APDownload protocol
IHC protocol for SAP130 antibody 12130-1-APDownload protocol
IF protocol for SAP130 antibody 12130-1-APDownload protocol
IP protocol for SAP130 antibody 12130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols