SAP130 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 12130-1-AP

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Freight/Packing

Con: 20 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells

Positive IP detected in:
HepG2 cells

Positive IHC detected in:
human pancreas cancer tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
Sin3A-associated protein, 130kDa

Calculated molecular weight:
110 kDa

Observed molecular weight:
130 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
SAP130

Synonyms
FLJ12761, SAP130
Background

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.


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