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  • KD/KO Validated

SEC24A Polyclonal antibody

SEC24A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15958-1-AP

Synonyms

SEC24 related protein A, SEC24A



Tested Applications

Positive WB detected inCOS-7 cells, HeLa cells, human liver tissue, mouse skeletal muscle tissue, mouse thymus tissue, rat thymus tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15958-1-AP targets SEC24A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC24A fusion protein Ag8723
Full Name SEC24 family, member A (S. cerevisiae)
Calculated Molecular Weight 613 aa, 66 kDa
Observed Molecular Weight 66 kDa, 120 kDa
GenBank Accession NumberBC019341
Gene Symbol SEC24A
Gene ID (NCBI) 10802
RRIDAB_2186118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). Two isoforms of 120 kDa and 66 kDa produced by alternative splicing have been described.

Protocols

Product Specific Protocols
WB protocol for SEC24A antibody 15958-1-APDownload protocol
IHC protocol for SEC24A antibody 15958-1-APDownload protocol
IF protocol for SEC24A antibody 15958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.

Authors - Jinbo Han
humanIF

Cell Metab

The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion.

Authors - Camilla Gustafsen
mouseWB

J Clin Invest

The COPII cargo adapter SEC24C is essential for neuronal homeostasis.

Authors - Bo Wang
mouseWB,IF

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
human,mouseWB,IF

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You
humanWB,IF,IP

J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li
  • KD Validated