SEC31A Antibody

SEC31A Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 17913-1-AP

Synonyms

ABP125, ABP130, HSPC275, HSPC334, KIAA0905, SEC31 like protein 1, SEC31 related protein A, SEC31A, SEC31L1, Web1 like protein



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17913-1-AP targets SEC31A in IF, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC31A fusion protein Ag12314
Full Name SEC31 homolog A (S. cerevisiae)
Calculated molecular weight 1220aa,133 kDa
Observed molecular weight 133 kDa
GenBank accession number BC084583
Gene symbol SEC31A
Gene ID (NCBI) 22872
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

Protocols

Product Specific Protocols
WB protocol for SEC31A antibody 17913-1-APDownload protocol
IF protocol for SEC31A antibody 17913-1-APDownload protocol
IP protocol for SEC31A antibody 17913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

PLoS Biol

Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning.

Authors - Carlos M Guardia
mouseWB

J Biol Chem

Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.

Authors - Xiaoling Li
ratWB

J Biol Chem

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Authors - Lee G D Fryer
ratWB

Cell Death Dis

ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons.

Authors - Tatiana Leiva-Rodríguez
mouseIF

Cell

Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

Authors - Moran Dvela-Levitt

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

RICHA GARVA (09-29-2019)

Clean, excellent and specific

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Protein from mouse tail tendon
SEC31A Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:17913-1-AP)
FH

Rui Yan (09-28-2019)

Labels intracellular vesicles with clear contrast. Cell monolayer fixed by 0.3% glutaraldehyde and 3% paraformaldehyde. Permeabilization: 0.1% saponin and 3% BSA in PBS. Primary antibody overnight at 4C.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Cos-7
SEC31A Antibody Immunofluorescence, validation (1:300 dilution) in Cos-7 (Cat no:17913-1-AP)