Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
17913-1-AP targets SEC31A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SEC31A fusion protein Ag12314 Predict reactive species |
| Full Name | SEC31 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 1220 aa, 133 kDa |
| Observed Molecular Weight | 118-134 kDa |
| GenBank Accession Number | BC084583 |
| Gene Symbol | SEC31A |
| Gene ID (NCBI) | 22872 |
| RRID | AB_2186378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94979 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC31A antibody 17913-1-AP | Download protocol |
| IF protocol for SEC31A antibody 17913-1-AP | Download protocol |
| IP protocol for SEC31A antibody 17913-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.
| ||
Cell Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy. | ||
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (09-01-2020) | This antibody labels a strong band around the expected molecular weight.
|
FH RICHA (Verified Customer) (09-29-2019) | Clean, excellent and specific
 |
FH Rui (Verified Customer) (09-28-2019) | Labels intracellular vesicles with clear contrast. Cell monolayer fixed by 0.3% glutaraldehyde and 3% paraformaldehyde. Permeabilization: 0.1% saponin and 3% BSA in PBS. Primary antibody overnight at 4C.
 |
