SEC31A Polyclonal Antibody for IF, IP, WB, ELISA
Cat no : 17913-1-AP
ABP125, ABP130, HSPC275, HSPC334, KIAA0905, SEC31 like protein 1, SEC31 related protein A, SEC31A, SEC31L1, Web1 like protein
|Positive WB detected in||HeLa cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17913-1-AP targets SEC31A in IF, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SEC31A fusion protein Ag12314|
|Full Name||SEC31 homolog A (S. cerevisiae)|
|Calculated molecular weight||1220aa,133 kDa|
|Observed molecular weight||133 kDa|
|GenBank accession number||BC084583|
|Gene ID (NCBI)||22872|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.
Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning.
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The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.
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ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
RICHA GARVA (09-29-2019)
Clean, excellent and specific
Rui Yan (09-28-2019)
Labels intracellular vesicles with clear contrast. Cell monolayer fixed by 0.3% glutaraldehyde and 3% paraformaldehyde. Permeabilization: 0.1% saponin and 3% BSA in PBS. Primary antibody overnight at 4C.