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SEC31A Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 17913-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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SEC31A Antibody WB HeLa cells 17913-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 17913-1-AP(SEC31A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

SEC31A Antibody IP HeLa cells 17913-1-AP

IP Result of anti-SEC31A (IP:17913-1-AP, 4ug; Detection:17913-1-AP 1:500) with HeLa cells lysate 1080ug.

SEC31A Antibody IF HeLa cells 17913-1-AP

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 17913-1-AP(SEC31A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, Jurkat cells
Positive IP detected in:
HeLa cells
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SEC31A fusion protein Ag12314
Full name:
SEC31 homolog A (S. cerevisiae)
Calculated molecular weight:
1220aa,133 kDa
Observed molecular weight:
133 kDa
GenBank accession number:
BC084583
Gene ID (NCBI):
22872
Gene symbol
SEC31A
Synonyms
ABP125, ABP130, HSPC275, HSPC334, KIAA0905, SEC31 like protein 1, SEC31 related protein A, SEC31A, SEC31L1, Web1 like protein

Background

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.


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