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CoraLite® Plus 488-conjugated SEC31A Polyclonal antibody

SEC31A Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17913

Synonyms

ABP125, ABP130, DKFZp686N07171, HSPC275, HSPC334, KIAA0905, SEC31 like protein 1, SEC31 related protein A, SEC31A, SEC31L1, Web1 like protein



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-17913 targets SEC31A in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC31A fusion protein Ag12314
Full Name SEC31 homolog A (S. cerevisiae)
Calculated molecular weight 1220 aa, 133 kDa
Observed molecular weight 133 kDa
GenBank accession numberBC084583
Gene symbol SEC31A
Gene ID (NCBI) 22872
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SEC31A antibody CL488-17913Download protocol
Standard Protocols
Click here to view our Standard Protocols