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SERPINI2 Polyclonal antibody

SERPINI2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13298-1-AP

Synonyms

MEPI, PANCPIN, Peptidase inhibitor 14, PI 14, PI14, Serpin I2, SERPINI2, TSA2004

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using SERPINI2 Polyclonal antibody (13298-1-AP)

    WB analysis of mouse pancreas using 13298-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13298-1-AP (SERPINI2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SERPINI2 Polyclonal antibody (13298-1-AP)

    IHC staining of mouse pancreas using 13298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13298-1-AP (SERPINI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SERPINI2 Polyclonal antibody (13298-1-AP)

    IHC staining of mouse pancreas using 13298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13298-1-AP (SERPINI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13298-1-AP targets SERPINI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINI2 fusion protein Ag4022
Full Name serpin peptidase inhibitor, clade I (pancpin), member 2
Calculated Molecular Weight 405 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC027859
Gene Symbol SERPINI2
Gene ID (NCBI) 5276
RRIDAB_2185341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SERPINI2 antibody 13298-1-APDownload protocol
IHC protocol for SERPINI2 antibody 13298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols