SIRT1 Antibody 31 Publications

Rabbit Polyclonal| Catalog number: 13161-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 45 μg/150 μl

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Species specificity:
human, mouse, zebrafish

Positive WB detected in:
HeLa cells, DU 145 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells

Positive IP detected in:
mouse testis tissue

Positive IHC detected in:
mouse testis tissue, human colon cancer tissue, human lung cancer tissue, mouse testis tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)

Calculated molecular weight:
747aa,82 kDa

Observed molecular weight:
110-130 kDa,80-85 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
SIRT1

Synonyms
hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1
Background

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, insulin receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.


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