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WB analysis using 13161-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 13161-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13161-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 13161-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13161-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13161-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 13161-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13161-1-AP (SIRT1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 13161-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13161-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (13161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HeLa using 13161-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13161-1-AP (SIRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SIRT1 Polyclonal antibody

SIRT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13161-1-AP

Publications(422)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

EC:2.3.1.-, EC:2.3.1.286, hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 750

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (422)
Protocols
Reviews (3)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, MDA-MB-231 cells
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 39 publications below
WB	See 384 publications below
IHC	See 49 publications below
IF	See 56 publications below
IP	See 10 publications below
ELISA	See 1 publications below
CoIP	See 10 publications below
RIP	See 1 publications below

Product Information

13161-1-AP targets SIRT1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, pig, chicken, zebrafish, bovine, sheep, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SIRT1 fusion protein Ag3808 Predict reactive species
Full Name	sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Calculated Molecular Weight	747 aa, 82 kDa
Observed Molecular Weight	110-130 kDa, 80-85 kDa
GenBank Accession Number	BC012499
Gene Symbol	SIRT1
Gene ID (NCBI)	23411
RRID	AB_10646436
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96EB6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT1, also named SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttle between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.

Protocols

Product Specific Protocols
WB protocol for SIRT1 antibody 13161-1-AP	Download protocol
IHC protocol for SIRT1 antibody 13161-1-AP	Download protocol
IF protocol for SIRT1 antibody 13161-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nature A plant-derived natural photosynthetic system for improving cell anabolism Authors - Pengfei Chen View Article
	WB	Cell Metab IgG is an aging factor that drives adipose tissue fibrosis and metabolic decline Authors - Lexiang Yu View Article
	WB,IF	Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. Authors - Yi Fu KD Validated View Article
	WB	Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection Authors - Yumei Que View Article
	WB,IHC	Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence Authors - Yinsheng Wu View Article
	IF	J Pineal Res Melatonin enhances mitochondrial biogenesis and protects against rotenone-induced mitochondrial deficiency in early porcine embryos. Authors - Ying-Jie Niu View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Tatyana (Verified Customer) (06-15-2025)	Incubation ON at 4C, in 5% goat serum in PBST. Gives primarily nuclear signal. Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: HeLa/human SIRT1 Antibody Immunofluorescence validation (1:1000 dilution) in HeLa/human (Cat no:13161-1-AP)
FH Uxoa (Verified Customer) (02-19-2020)	Non nuclear signal of SIRT1 in human fibroblast. Cells fixed with 4% PFA 15 min. Ab dilution 1:100 in PBST (0.1% triton)+10% GS. O/N 4ºC incubation. Secondary ab: Goat anti-rabbit alexafluor 555 1:500 in PBST + 10% GS 1h RT. Applications: Immunofluorescence, Primary Antibody Dilution: 1:100 Cell Tissue Type: Human fibroblasts SIRT1 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:13161-1-AP)
FH Tanusree (Verified Customer) (12-18-2019)	Product worked well in WB at 1:500 dilution Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: Mouse brain