SIRT1 Antibody 13161-1-AP

Tested Applications

Applications:

ELISA, IF, IHC, IP, WB
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HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP( SIRT1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

IP Result of anti-SIRT1 (IP:13161-1-AP, 4ug; Detection:13161-1-AP 1:500) with mouse testis tissue lysate 8000ug.

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13161-1-AP(SIRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, zebrafish

Positive WB detected in:

HeLa cells, DU 145 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells

Positive IP detected in:

mouse testis tissue

Positive IHC detected in:

mouse testis tissue, human colon cancer tissue, human lung cancer tissue, mouse testis tissue

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:50-1:200

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

SIRT1 fusion protein Ag3808

Full name:

sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)

Calculated molecular weight:

747aa,82 kDa

Observed molecular weight:

110-130 kDa,80-85 kDa

GenBank accession number:

BC012499

Gene ID (NCBI):

23411

Gene symbol

SIRT1

Synonyms

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

Background

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, insulin receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.

Showing 1- [6] of [31] Publications

Author:		Endo Hitoshi H
Pubmed ID:		23696823
Journal:		PLoS One
Title:		Butyrate-producing probiotics reduce nonalcoholic fatty liver disease progression in rats: new insight into the probiotics for the gut-liver axis.
Application:		WB
KD validated
Species:		human

Author:		Haranatha R Potteti
Pubmed ID:		26465873
Journal:		Am J Respir Cell Mol Biol
Title:		Sirtuin 1 Promotes Hyperoxia-induced Lung Epithelial Death Independent of NRF2 Activation.
Application:		WB
KD validated
Species:		human

Author:		Lisha Zhao
Pubmed ID:		27455216
Journal:		Molecules
Title:		Protective Effect of the Total Flavonoids from Rosa laevigata Michx Fruit on Renal Ischemia-Reperfusion Injury through Suppression of Oxidative Stress and Inflammation.
Application:		WB
KD validated
Species:		rat

Author:		Li Jia
Pubmed ID:		27824080
Journal:		Sci Rep
Title:		Lycium barbarum polysaccharide attenuates high-fat diet-induced hepatic steatosis by up-regulating SIRT1 expression and deacetylase activity.
Application:		WB
KD validated
Species:		human

Author:		P Zhang
Pubmed ID:		27931585
Journal:		Transplant Proc
Title:		Resveratrol Ameliorated Vascular Calcification by Regulating Sirt-1 and Nrf2.
Application:		WB

Species:		human

Author:		Chao Zhang
Pubmed ID:		28112228
Journal:		Sci Rep
Title:		Hormetic effect of panaxatriol saponins confers neuroprotection in PC12 cells and zebrafish through PI3K/AKT/mTOR and AMPK/SIRT1/FOXO3 pathways.
Application:		WB

Species:		human

WB Figures

DU 145 cells were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP( SIRT1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PC-3 cells were subjected to SDS PAGE followed by western blot with 13161-1-AP(SIRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-SIRT1 (IP:13161-1-AP, 4ug; Detection:13161-1-AP 1:500) with mouse testis tissue lysate 8000ug.

IF Figures

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13161-1-AP(SIRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

SIRT1 Antibody 31 Publications

Rabbit Polyclonal| Catalog number: 13161-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [31] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

SIRT1 Antibody 31 Publications

Rabbit Polyclonal| Catalog number: 13161-1-AP

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Tested Applications

Product Information

Immunogen Information

Background