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SIRT1 Antibody 49 Publications

Rabbit Polyclonal| Catalog number: 13161-1-AP

Featured Product KD/KO validated

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

SIRT1 Antibody WB HeLa cells 13161-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP( SIRT1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

SIRT1 Antibody IP mouse testis tissue 13161-1-AP

IP Result of anti-SIRT1 (IP:13161-1-AP, 4ug; Detection:13161-1-AP 1:500) with mouse testis tissue lysate 8000ug.

SIRT1 Antibody IHC mouse testis tissue 13161-1-AP

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 40x lens)

SIRT1 Antibody IF HeLa cells 13161-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13161-1-AP(SIRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, Blunt snout bream, mouse, Zebrafish
Positive WB detected in:
HeLa cells
Positive IP detected in:
mouse testis tissue
Positive IHC detected in:
mouse testis tissue, human colon cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SIRT1 fusion protein Ag3808
Full name:
sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Calculated molecular weight:
747aa,82 kDa
Observed molecular weight:
110-130 kDa,80-85 kDa
GenBank accession number:
BC012499
Gene ID (NCBI):
23411
Gene symbol
SIRT1
Synonyms
hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

Background

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, insulin receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.


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