SLC1A4 Polyclonal antibody

SLC1A4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13067-2-AP

Synonyms

ASCT 1, ASCT1, SATT, SLC1A4



Tested Applications

Positive WB detected inRaji cells, mouse brain tissue, human skeletal muscle tissue, SW480 cells, mouse kidney tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

13067-2-AP targets SLC1A4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC1A4 fusion protein Ag3763
Full Name solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
Calculated Molecular Weight 532 aa, 56 kDa
Observed Molecular Weight 62-70 kDa
GenBank Accession NumberBC026216
Gene Symbol SLC1A4
Gene ID (NCBI) 6509
RRIDAB_2190604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC1A4 antibody 13067-2-APDownload protocol
IHC protocol for SLC1A4 antibody 13067-2-APDownload protocol
IP protocol for SLC1A4 antibody 13067-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner

Authors - Xin Liu
mouseWB

Ecotoxicol Environ Saf

Toxic effects of maternal cadmium exposure on the metabolism and transport system of amino acids in the maternal livers

Authors - Peng Xu
humanWB

Front Oncol

SLC1A4: A Powerful Prognostic Marker and Promising Therapeutic Target for HCC.

Authors - Xiaozhen Peng
mouseWB

Res Sq

Generation and characterization of a knock-in mouse model for Spastic Tetraplegia, Thin Corpus Callosum, and Progressive Microcephaly (SPATCCM)

Authors - Megan L Ratz