• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SLC22A2 Polyclonal antibody

SLC22A2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13594-1-AP

Synonyms

hOCT2, OCT2, Organic cation transporter 2, SLC22A2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SLC22A2 Polyclonal antibody (13594-1-AP)

    WB analysis using 13594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13594-1-AP (SLC22A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC22A2 Polyclonal antibody (13594-1-AP)

    WB analysis of HEK-293 using 13594-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13594-1-AP (SLC22A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13594-1-AP targets SLC22A2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC22A2 fusion protein Ag4500
Full Name solute carrier family 22 (organic cation transporter), member 2
Calculated Molecular Weight 555 aa, 63 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC039899
Gene Symbol SLC22A2
Gene ID (NCBI) 6582
RRIDAB_2239467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC22A2, also named as OCT2, belongs to the major facilitator (TC 2.A.1) superfamily and Organic cation transporter (TC 2.A.1.19) family. The SLC22A2 gene encodes for the organic cation transporter 2 (OCT2) protein which acts as a renal uptake transporter. Human OCT2 (hOCT2) is expressed on the basolateral side of the proximal tubule cells and plays a crucial role in the disposition and renal clearance of cationic drugs and endogenous compounds (PMID: 35143948). SLC22A2 has 3 isoforms with the molecular mass of 27, 54 and 63 kDa. Sometimes higher molecular weight around 70 kDa can also be observed, which may be a modified variant of SLC22A2.

Protocols

Product Specific Protocols
WB protocol for SLC22A2 antibody 13594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Organic cation transporter 2 activation enhances sensitivity to oxaliplatin in human pancreatic ductal adenocarcinoma

Authors - Ching-Feng Chiu
View Article
human,mouseWB

J Pharm Anal

Discovering metabolic vulnerability using spatially resolved metabolomics for antitumor small molecule-drug conjugates development as a precise cancer therapy strategy

Authors - Xiangyi Wang
View Article