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SLC25A12 Polyclonal antibody

SLC25A12 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26804-1-AP

Synonyms

AGC1, ARALAR, ARALAR1, SLC25A12

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    WB analysis of mouse skeletal muscle using 26804-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    WB analysis of mouse kidney using 26804-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC25A12 Polyclonal antibody (26804-1-AP)

    WB analysis using 26804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human breast cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human breast cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human pancreas cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human pancreas cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human stomach cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of human stomach cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of mouse kidney using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IHC staining of mouse kidney using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using SLC25A12 Polyclonal antibody (26804-1-AP)

    IF Staining of mouse heart using 26804-1-AP

    Immunofluorescent analysis of (4% PFA) fixed tissue using 26804-1-AP (SLC25A12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26804-1-AP targets SLC25A12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A12 fusion protein Ag25150
Full Name solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Calculated Molecular Weight 75 kDa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC016932
Gene Symbol SLC25A12
Gene ID (NCBI) 8604
RRIDAB_2880641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC25A12 antibody 26804-1-APDownload protocol
IHC protocol for SLC25A12 antibody 26804-1-APDownload protocol
IF protocol for SLC25A12 antibody 26804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols