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SLC25A15 Polyclonal antibody

SLC25A15 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15317-1-AP

Synonyms

D13S327, HHH, ORC1, ORNT1, SLC25A15

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SLC25A15 Polyclonal antibody (15317-1-AP)

    WB analysis of HeLa using 15317-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    WB analysis of mouse liver using 15317-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    WB analysis of mouse stomach using 15317-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human liver cancer using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human liver cancer using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human liver cancer using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human liver cancer using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human skin using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human skin using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human brain using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human brain using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human lung using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human lung using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human ovary using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SLC25A15 Polyclonal antibody (15317-1-AP)

    IHC staining of human ovary using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SLC25A15 Polyclonal antibody (15317-1-AP)

    IF Staining of MCF-7 using 15317-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody (15317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse stomach tissue
Positive IHC detected inhuman liver cancer tissue, human skin tissue, human brain tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15317-1-AP targets SLC25A15 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A15 fusion protein Ag7563
Full Name solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC002702
Gene Symbol SLC25A15
Gene ID (NCBI) 10166
RRIDAB_2189758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa.

Protocols

Product Specific Protocols
WB protocol for SLC25A15 antibody 15317-1-APDownload protocol
IHC protocol for SLC25A15 antibody 15317-1-APDownload protocol
IF protocol for SLC25A15 antibody 15317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols