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SLC25A18 Polyclonal antibody

SLC25A18 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17348-1-AP

Synonyms

GC 2, GC2, Glutamate/H(+) symporter 2, SLC25A18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A18 Polyclonal antibody (17348-1-AP)

    WB analysis using 17348-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17348-1-AP (SLC25A18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC25A18 Polyclonal antibody (17348-1-AP)

    WB analysis of mouse kidney using 17348-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17348-1-AP (SLC25A18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SLC25A18 Polyclonal antibody (17348-1-AP)

    IP experiment of mouse brain using 17348-1-AP

    IP result of anti-SLC25A18 (IP:17348-1-AP, 4ug; Detection:17348-1-AP 1:500) with mouse brain tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A18 Polyclonal antibody (17348-1-AP)

    IHC staining of mouse brain using 17348-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17348-1-AP (SLC25A18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A18 Polyclonal antibody (17348-1-AP)

    IHC staining of mouse brain using 17348-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17348-1-AP (SLC25A18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17348-1-AP targets SLC25A18 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A18 fusion protein Ag11420
Full Name solute carrier family 25 (mitochondrial carrier), member 18
Calculated Molecular Weight 315 aa, 34 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC031644
Gene Symbol SLC25A18
Gene ID (NCBI) 83733
RRIDAB_2189889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A18, also named as GC 2, belongs to the mitochondrial transporter family (SLC25). It is located in inner mitochondrial membrane which involved in the transport of glutamate either with H+ or in exchange for OH-. SLC25A18 is highly expressed in brain, testis, lung, kidney, liver, spleen and skeletal muscle. 17348-1-AP antibody detects the band around 32-35 kDa in SDS-PAGE.   (PMID: 14598172)

Protocols

Product Specific Protocols
WB protocol for SLC25A18 antibody 17348-1-APDownload protocol
IHC protocol for SLC25A18 antibody 17348-1-APDownload protocol
IP protocol for SLC25A18 antibody 17348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Cancer Res

SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling.

Authors - Li Liang
View Article