SLC25A22 Polyclonal antibody

SLC25A22 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25402-1-AP

Synonyms

GC 1, GC1, Glutamate/H(+) symporter 1, NET44, SLC25A22



Tested Applications

Positive WB detected inmouse brain tissue, Caco-2 cells, HT-29 cells, rat brain tissue, NCI-H1299 cells
Positive IHC detected inmouse brain tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOS-7 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25402-1-AP targets SLC25A22 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A22 fusion protein Ag19958
Full Name solute carrier family 25 (mitochondrial carrier: glutamate), member 22
Calculated Molecular Weight 323 aa, 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC023545
Gene Symbol SLC25A22
Gene ID (NCBI) 79751
RRIDAB_2880060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)

Protocols

Product Specific Protocols
WB protocol for SLC25A22 antibody 25402-1-APDownload protocol
IHC protocol for SLC25A22 antibody 25402-1-APDownload protocol
IF protocol for SLC25A22 antibody 25402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Dual-process brain mitochondria isolation preserves function and clarifies protein composition.

Authors - Maria F Noterman