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GLUT1 Antibody

GLUT1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21829-1-AP

Synonyms

DYT17, DYT18, GLUT, GLUT 1, GLUT1, HepG2 glucose transporter, PED, SLC2A1



Tested Applications

Positive WB detected inY79 cells, HEK-293 cells, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 21829-1-AP immunogen is Fusion Protein GLUT1 protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLUT1 fusion protein Ag16282
Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Calculated molecular weight 492aa,54 kDa
Observed molecular weight 45-55 kDa
GenBank accession number BC121804
Gene symbol SLC2A1
Gene ID (NCBI) 6513
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate.

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.



Protocols

Product Specific Protocols
WB protocol for GLUT1 antibody 21829-1-APDownload protocol
IHC protocol for GLUT1 antibody 21829-1-APDownload protocol
IF protocol for GLUT1 antibody 21829-1-APDownload protocol
FC protocol for GLUT1 antibody 21829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.

Authors - Erhui Jiang
humanIHC

Contrast Media Mol Imaging

Fructose 1,6-Bisphosphatase 1 Expression Reduces 18F-FDG Uptake in Clear Cell Renal Cell Carcinoma.

Authors - Ruohua Chen
humanWB

Cancer Res

Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer.

Authors - Dalong Cao
humanWB

Nat Commun

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Authors - Biao Ma
human

Cell Metab

High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.

Authors - Chun-Mei Hu
humanWB

Autophagy

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Authors - Xing Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Susan (Verified Customer) (11-19-2019)

20ug of HeLa cells overexpressing Glut1-GFP. Blocked with 5% milk in 0.1% TBST and incubated overnight at 4 degrees with rocking.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
GLUT1 Antibody Western Blot,Immunofluorescence, validation (1:2000 dilution) in HeLa (Cat no:21829-1-AP)
FH

Kishor (Verified Customer) (12-13-2018)

I got good results in cultured cells but I could not see any results with rat liver protein.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
No image submitted with review