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GLUT2 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 20436-1-AP

Featured Product KD/KO validated

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Con: 33 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

GLUT2 Antibody WB HEK 293 cells 20436-1-AP

WB result of GLUT2 antibody (20436-1-AP, 1:300) with si-Control and si-GLUT2 transfected HEK 293 cells

GLUT2 Antibody IHC human small intestine tissue 20436-1-AP

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 20436-1-AP( GLUT2 antibody at dilution of 1:500 (under 40x lens).

GLUT2 Antibody IHC human small intestine tissue 20436-1-AP

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 20436-1-AP( GLUT2 antibody at dilution of 1:500 (under 10x lens).

GLUT2 Antibody WB rat liver tissue 20436-1-AP

rat liver tissue were subjected to SDS PAGE followed by western blot with 20436-1-AP( GLUT2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

GLUT2 Antibody IP mouse kidney tissue 20436-1-AP

IP Result of anti-GLUT2 (IP:20436-1-AP, 4ug; Detection:20436-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

Species specificity:
human, rat, mouse
Positive WB detected in:
rat liver tissue, HEK 293 cells
Positive IP detected in:
mouse kidney tissue
Positive IHC detected in:
human small intestine tissue
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:1000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GLUT2 fusion protein Ag14204
Full name:
solute carrier family 2 (facilitated glucose transporter), member 2
Calculated molecular weight:
351aa,39 kDa
Observed molecular weight:
60-70 kDa, 38-45 kDa
GenBank accession number:
BC060041
Gene ID (NCBI):
6514
Gene symbol
SLC2A2
Synonyms
GLUT 2, GLUT2, SLC2A2

Background

GLUT2 is a member of glucose transporters or GLUTs which catalyze the glucose transport across plasma membrane. GLUT2 is selectively expressed in hepatocytes, pancreatic beta cells, and absorptive renal and intestinal epithelial cells. GLUT2 is required to maintain normal glucose homeostasis and normal function and development of the endocrine pancreas. Its absence leads to symptoms characteristic of non-insulin-dependent diabetes mellitus. This antibody detected 60-70 kDa fully glycosylated, matured GLUT2 and a nonglycosylated form of GLUT2 around 38-45 kDa. (9354799, 12101013)


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