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SMAD5 Polyclonal antibody

SMAD5 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12167-1-AP

Synonyms

Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12167-1-AP targets SMAD5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD5 fusion protein Ag2809
Full Name SMAD family member 5
Calculated molecular weight 465 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009682
Gene symbol SMAD5
Gene ID (NCBI) 4090
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Protocols

Product Specific Protocols
WB protocol for SMAD5 antibody 12167-1-APDownload protocol
IF protocol for SMAD5 antibody 12167-1-APDownload protocol
IP protocol for SMAD5 antibody 12167-1-APDownload protocol
FC protocol for SMAD5 antibody 12167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.

Authors - Jinliang Chen

PLoS Biol

BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.

Authors - Christian Hiepen
ratWB

Sci Rep

Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.

Authors - Yan Zeng
humanWB

Life Sci

Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.

Authors - Rui Mu
mouseWB

PLoS One

Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.

Authors - Zhang Xing-Yi XY
mouseWB

Sci Rep

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Authors - Xueni Wang