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STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11459-1-AP

Synonyms

EIEE4, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A



Tested Applications

Positive WB detected inHeLa cells, A549 cells, A375 cells, human brain tissue, Y79 cells, Jurkat cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11459-1-AP targets STXBP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STXBP1 fusion protein Ag1978
Full Name syntaxin binding protein 1
Calculated Molecular Weight 594 aa, 68 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_2196690
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STXBP1 antibody 11459-1-APDownload protocol
IHC protocol for STXBP1 antibody 11459-1-APDownload protocol
IF protocol for STXBP1 antibody 11459-1-APDownload protocol
IP protocol for STXBP1 antibody 11459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IHC

EMBO J

Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.

Authors - Xianping Wang
mouseWB, IF

Proc Natl Acad Sci U S A

Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.

Authors - Poppy Datta
mouseWB

J Biol Chem

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

Authors - Poppy Datta
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
mouseWB, IHC

PLoS One

Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.

Authors - Poppy Datta
mouseWB

Cell Rep

Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies

Authors - Nadeem Murtaza