Biotin-conjugated STXBP1 Polyclonal antibody

• 

  WB analysis of Y79 using Biotin-11459

  Y79 cells were subjected to SDS PAGE followed by western blot with Biotin-11459 (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using Biotin-11459

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using Biotin-11459

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

STXBP1 Polyclonal Antibody for WB, IHC

Cat No. Biotin-11459

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Synonyms

EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

---

Tested Applications

• Positive WB detected in: Y79 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-11459 targets STXBP1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1978

  Product name: Recombinant human STXBP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 245-594 aa of BC015749

  Sequence: HELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS

• Full Name: syntaxin binding protein 1
• Calculated Molecular Weight: 594 aa, 68 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC015749
• Gene Symbol: STXBP1
• Gene ID (NCBI): 6812
• RRID: AB_2918937
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61764
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.