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  • Featured Product
  • KD/KO Validated

SUB1 Polyclonal antibody

SUB1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11956-1-AP

Synonyms

p14, P15, PC4, Positive cofactor 4, RPO2TC1, SUB1, SUB1 homolog, SUB1 homolog (S. cerevisiae)

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using SUB1 Polyclonal antibody (11956-1-AP)

    WB analysis of rat liver using 11956-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 11956-1-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SUB1 Polyclonal antibody (11956-1-AP)

    WB analysis of HeLa using 11956-1-AP

    WB result of SUB1 antibody (11956-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected HeLa cells.

  • Western Blot (WB) analysis of C2C12 cells using SUB1 Polyclonal antibody (11956-1-AP)

    WB analysis of C2C12 using 11956-1-AP

    C2C12 cell were subjected to SDS PAGE followed by western blot with 11956-1-AP (SUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SUB1 Polyclonal antibody (11956-1-AP)

    WB analysis of HeLa using 11956-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11956-1-AP (SUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SUB1 Polyclonal antibody (11956-1-AP)

    IHC staining of human gliomas using 11956-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11956-1-AP (SUB1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SUB1 Polyclonal antibody (11956-1-AP)

    IHC staining of human gliomas using 11956-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11956-1-AP (SUB1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUB1 Polyclonal antibody (11956-1-AP)

    IF Staining of HeLa using 11956-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11956-1-AP (SUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inrat liver tissue, HeLa cells, C2C12 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11956-1-AP targets SUB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUB1 fusion protein Ag2559
Full Name SUB1 homolog (S. cerevisiae)
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 18-23 kDa
GenBank Accession NumberBC022339
Gene Symbol SUB1
Gene ID (NCBI) 10923
RRIDAB_2197148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#11956-1-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).

Protocols

Product Specific Protocols
WB protocol for SUB1 antibody 11956-1-APDownload protocol
IHC protocol for SUB1 antibody 11956-1-APDownload protocol
IF protocol for SUB1 antibody 11956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols