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CD134/OX40 Polyclonal antibody

CD134/OX40 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 20006-1-AP

Synonyms

ACT35, ACT35 antigen, CD134, CD134/OX40, OX40, OX40L receptor, TNFRSF4, TXGP1L

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CD134/OX40 Polyclonal antibody (20006-1-AP)

    WB analysis of HepG2 using 20006-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using CD134/OX40 Polyclonal antibody (20006-1-AP)

    WB analysis of L02 using 20006-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD134/OX40 Polyclonal antibody (20006-1-AP)

    WB analysis of U-937 using 20006-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of human PBMCs using CD134/OX40 Polyclonal antibody (20006-1-AP)

    FC experiment of human PBMCs using 20006-1-AP

    1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or unstained (blue). Unstimulated human PBMCs were surface stained with 0.2ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, U-937 cells
Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20006-1-AP targets CD134/OX40 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tumor necrosis factor receptor superfamily, member 4
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberNM_003327
Gene Symbol TNFRSF4
Gene ID (NCBI) 7293
RRIDAB_10733472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.

Protocols

Product Specific Protocols
WB protocol for CD134/OX40 antibody 20006-1-APDownload protocol
FC protocol for CD134/OX40 antibody 20006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

A Novel Immune-Related Gene Signature to Identify the Tumor Microenvironment and Prognose Disease Among Patients With Oral Squamous Cell Carcinoma Patients Using ssGSEA: A Bioinformatics and Biological Validation Study

Authors - Yun Chen
View Article
humanWB

Dis Markers

Development of a Costimulatory Molecule Signature to Predict Prognosis, Immune Landscape, and Response to Immune Therapy for Hepatocellular Carcinoma

Authors - Yongjie Zhou
View Article