CD86 Polyclonal antibody

CD86 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 13395-1-AP

Synonyms

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, FUN 1, LAB72



Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IF detected inraji cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13395-1-AP targets CD86 in WB, IF, FC, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD86 fusion protein Ag4063
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC040261
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).

Protocols

Product Specific Protocols
WB protocol for CD86 antibody 13395-1-APDownload protocol
IF protocol for CD86 antibody 13395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Biomaterials

Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.

Authors - Haoran Wang
mouseFC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
humanWB,IF

Theranostics

TGFBI secreted by tumor-associated macrophages promotes glioblastoma stem cell-driven tumor growth via integrin αvβ5-Src-Stat3 signaling.

Authors - Peng Peng
ratWB

Theranostics

Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.

Authors - Luodan Yang
mouseIF

Cell Mol Immunol

MEF2C promotes M1 macrophage polarization and Th1 responses.

Authors - Xibao Zhao
ratWB

Neurobiol Stress

Microglia-derived TNF-α inhibiting GABAergic neurons in the anterior lateral bed nucleus of the stria terminalis precipitates visceral hypersensitivity induced by colorectal distension in rats.

Authors - Ning-Ning Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Martin (Verified Customer) (09-28-2022)

Antibody is working very well

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:300