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CD86 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 13395-1-AP

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Con: 28 μg/150 μl

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Tested Applications

Applications:
WB, IF, FC, ELISA
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Images See all

CD86 Antibody WB Raji cells 13395-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 13395-1-AP (CD86 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

CD86 Antibody FC Raji cells 13395-1-AP

1X10^6 Raji cells were stained with 0.2ug CD86 antibody (13395-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, rat
Positive WB detected in:
Raji cells
Positive FC detected in:
Raji cells
Recommended dilution:
WB : 1:500-1:1000
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD86 fusion protein Ag4063
Full name:
CD86 molecule
Calculated molecular weight:
329aa,38 kDa
Observed molecular weight:
65-70 kDa
GenBank accession number:
BC040261
Gene ID (NCBI):
942
Gene symbol
CD86
Synonyms
Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, FUN 1, LAB72

Background

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).


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