CD86 Polyclonal antibody
CD86 Polyclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IF, FC, Cell treatment, ELISA
Conjugate
Unconjugated
Cat no : 13395-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Raji cells |
| Positive IF detected in | raji cells |
| Positive FC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 22 publications below |
| IHC | See 9 publications below |
| IF | See 25 publications below |
| FC | See 5 publications below |
Product Information
13395-1-AP targets CD86 in WB, IF, FC, Cell treatment, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD86 fusion protein Ag4063 |
| Full Name | CD86 molecule |
| Calculated molecular weight | 329 aa, 38 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC040261 |
| Gene symbol | CD86 |
| Gene ID (NCBI) | 942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD86 antibody 13395-1-AP | Download protocol |
| IF protocol for CD86 antibody 13395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuropsychiatr Dis Treat Soluble epoxide hydrolase inhibition enhances anti-inflammatory and antioxidative processes, modulates microglia polarization, and promotes recovery after ischemic stroke. | ||
Cell Death Dis EB virus-induced ATR activation accelerates nasopharyngeal carcinoma growth via M2-type macrophages polarization. | ||
Acta Biomater Bacterial navigation for tumor targeting and photothermally-triggered bacterial ghost transformation for spatiotemporal drug release. | ||
Aging Cell Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells. | ||
ACS Appl Mater Interfaces Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy. | ||
Cell Death Discov A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression. |
